Targeting pancreatic cancer with magneto-fluorescent theranostic gold nanoshells

Wenxue Chen, Ciceron Ayala-Orozco, Nrusingh C. Biswal, Carlos Perez-Torres, Marc Bartels, Rizia Bardhan, Gary Stinnet, Xian De Liu, Baoan D Ji, Amit Deorukhkar, Lisa V. Brown, Sushovan Guha, Robia G. Pautler, Sunil Krishnan, Naomi J. Halas, Amit Joshi

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Aim: We report a magneto-fluorescent theranostic nanocomplex targeted to neutrophil gelatinase-associated lipocalin (NGAL) for imaging and therapy of pancreatic cancer. Materials & methods: Gold nanoshells resonant at 810 nm were encapsulated in silica epilayers doped with iron oxide and the near-infrared (NIR) dye indocyanine green, resulting in theranostic gold nanoshells (TGNS), which were subsequently conjugated with antibodies targeting NGAL in AsPC-1-derived xenografts in nude mice. Results: Anti-NGAL-conjugated TGNS specifically targeted pancreatic cancer cells in vitro and in vivo providing contrast for both NIR fluorescence and T2-weighted MRI with higher tumor contrast than can be obtained using long-circulating, but nontargeted, PEGylated nanoparticles. The nanocomplexes also enabled highly specific cancer cell death via NIR photothermal therapy in vitro. Conclusion: TGNS with embedded NIR and magnetic resonance contrasts can be specifically targeted to pancreatic cancer cells with expression of early disease marker NGAL, and enable molecularly targeted imaging and photothermal therapy.

Original languageEnglish (US)
Pages (from-to)1209-1222
Number of pages14
JournalNanomedicine
Volume9
Issue number8
DOIs
StatePublished - 2014

Fingerprint

Nanoshells
Lipocalins
Gelatinases
Pancreatic Neoplasms
gold
Gold
targeting
cancer
near infrared
Infrared radiation
Cells
Imaging techniques
Indocyanine Green
Epilayers
Cell death
Magnetic resonance
Iron oxides
Heterografts
Nude Mice
Antibodies

Keywords

  • MRI
  • nanoshells
  • noninvasive imaging
  • optical imaging
  • pancreatic cancer
  • photothermal

ASJC Scopus subject areas

  • Materials Science(all)
  • Bioengineering
  • Biomedical Engineering
  • Medicine (miscellaneous)
  • Development

Cite this

Chen, W., Ayala-Orozco, C., Biswal, N. C., Perez-Torres, C., Bartels, M., Bardhan, R., ... Joshi, A. (2014). Targeting pancreatic cancer with magneto-fluorescent theranostic gold nanoshells. Nanomedicine, 9(8), 1209-1222. https://doi.org/10.2217/nnm.13.84

Targeting pancreatic cancer with magneto-fluorescent theranostic gold nanoshells. / Chen, Wenxue; Ayala-Orozco, Ciceron; Biswal, Nrusingh C.; Perez-Torres, Carlos; Bartels, Marc; Bardhan, Rizia; Stinnet, Gary; Liu, Xian De; Ji, Baoan D; Deorukhkar, Amit; Brown, Lisa V.; Guha, Sushovan; Pautler, Robia G.; Krishnan, Sunil; Halas, Naomi J.; Joshi, Amit.

In: Nanomedicine, Vol. 9, No. 8, 2014, p. 1209-1222.

Research output: Contribution to journalArticle

Chen, W, Ayala-Orozco, C, Biswal, NC, Perez-Torres, C, Bartels, M, Bardhan, R, Stinnet, G, Liu, XD, Ji, BD, Deorukhkar, A, Brown, LV, Guha, S, Pautler, RG, Krishnan, S, Halas, NJ & Joshi, A 2014, 'Targeting pancreatic cancer with magneto-fluorescent theranostic gold nanoshells', Nanomedicine, vol. 9, no. 8, pp. 1209-1222. https://doi.org/10.2217/nnm.13.84
Chen W, Ayala-Orozco C, Biswal NC, Perez-Torres C, Bartels M, Bardhan R et al. Targeting pancreatic cancer with magneto-fluorescent theranostic gold nanoshells. Nanomedicine. 2014;9(8):1209-1222. https://doi.org/10.2217/nnm.13.84
Chen, Wenxue ; Ayala-Orozco, Ciceron ; Biswal, Nrusingh C. ; Perez-Torres, Carlos ; Bartels, Marc ; Bardhan, Rizia ; Stinnet, Gary ; Liu, Xian De ; Ji, Baoan D ; Deorukhkar, Amit ; Brown, Lisa V. ; Guha, Sushovan ; Pautler, Robia G. ; Krishnan, Sunil ; Halas, Naomi J. ; Joshi, Amit. / Targeting pancreatic cancer with magneto-fluorescent theranostic gold nanoshells. In: Nanomedicine. 2014 ; Vol. 9, No. 8. pp. 1209-1222.
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