Targeting of the creatine kinase M gene in embryonic stem cells using isogenic and nonisogenic vectors

Jan Van Deursen, B. Wieringa

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Replacement vectors with genomic DNA originating from different mouse strains were used to introduce site-specific mutations into the creatine kinase M (CKM) gene of mouse embryonic stem (ES) cells. Here we demonstrate that in mouse strain 129-derived ES cells, the gene is at least 25-fold more efficiently targeted with an isogenic, 129-derived vector (129-pRV8.3) than with a nonisogenic, BALB/c-specific vector (BALB/cpRV8.3). The two targeting constructs were identical except for allelic differences which were typed by partial sequencing. These included base pair mismatches (2%) and a polymorphic [GTC]-repeat length variation. Both in separate transfections as well as in cotransfections with mixed vectors, homologous disruption of the CKM gene resulted uniquely from the 129-isogenic DNA. Our data confirm earlier observations on requirements for homologous recombination in pro- and eukaryotic systems and indicate that targeting of the CKM locus is highly sensitive to small sequence differences between cognate segments in the endogenous and incoming DNA.

Original languageEnglish (US)
Pages (from-to)3815-3820
Number of pages6
JournalNucleic Acids Research
Volume20
Issue number15
StatePublished - 1992
Externally publishedYes

Fingerprint

MM Form Creatine Kinase
Stem Cells
Embryonic Stem Cells
Stem cells
Genes
Gene
Mouse
DNA
Minor Lymphocyte Stimulatory Loci
Base Pair Mismatch
129 Strain Mouse
Homologous Recombination
Creatine Kinase
Difference Sequence
Transfection
Recombination
Sequencing
Replacement
Genomics
Locus

ASJC Scopus subject areas

  • Genetics
  • Statistics, Probability and Uncertainty
  • Health, Toxicology and Mutagenesis
  • Applied Mathematics
  • Genetics(clinical)
  • Toxicology

Cite this

Targeting of the creatine kinase M gene in embryonic stem cells using isogenic and nonisogenic vectors. / Van Deursen, Jan; Wieringa, B.

In: Nucleic Acids Research, Vol. 20, No. 15, 1992, p. 3815-3820.

Research output: Contribution to journalArticle

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