Targeted Sequencing Study to Uncover Shared Genetic Susceptibility Between Peripheral Artery Disease and Coronary Heart Disease-Brief Report

Maya S. Safarova, Xiao Fan, Erin E. Austin, Natalie van Zuydam, Jemma Hopewell, Daniel J Schaid, Iftikhar Jan Kullo

Research output: Contribution to journalArticle

Abstract

Objective- It is unclear to what extent genetic susceptibility variants are shared between peripheral artery disease (PAD) and coronary heart disease (CHD), both manifestations of atherosclerotic vascular disease. We investigated whether common and low-frequency/rare variants in loci associated with CHD are also associated with PAD. Approach and Results- Targeted sequencing of 41 genomic regions associated with CHD in genome-wide association studies was performed in 1749 PAD cases (65±11 years, 61% men) and 1855 controls (60±11 years, 56% men) of European ancestry. PAD cases had a resting/postexercise ankle-brachial index ≤0.9, or history of lower extremity revascularization; controls had no history of PAD. We tested the association of common (defined as minor allele frequency ≥5%) variants with PAD assuming an additive genetic model with adjustment for age and sex. To identify low-frequency/rare variants (minor allele frequency <5%) associated with PAD, we conducted gene-level analyses using sequence kernel association test and permutation test. After Bonferroni correction, we found common variants in SH2B3, ABO, and ZEB2 to be associated with PAD ( P<4.5×10-5). At the gene level, the strongest associations were for LPL and SH2B3. Conclusions- Targeted sequencing of 41 genomic regions associated with CHD revealed several common variants/genes to be associated with PAD, highlighting the basis of shared genetic susceptibility between CHD and PAD.

Original languageEnglish (US)
Pages (from-to)1227-1233
Number of pages7
JournalArteriosclerosis, thrombosis, and vascular biology
Volume39
Issue number6
DOIs
StatePublished - Jun 1 2019

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Peripheral Arterial Disease
Genetic Predisposition to Disease
Coronary Disease
Gene Frequency
Genes
Ankle Brachial Index
Genome-Wide Association Study
Genetic Models
Vascular Diseases
Sequence Analysis
Lower Extremity

Keywords

  • atherosclerosis
  • exome
  • genotype
  • lower extremity
  • untranslated regions

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Targeted Sequencing Study to Uncover Shared Genetic Susceptibility Between Peripheral Artery Disease and Coronary Heart Disease-Brief Report. / Safarova, Maya S.; Fan, Xiao; Austin, Erin E.; van Zuydam, Natalie; Hopewell, Jemma; Schaid, Daniel J; Kullo, Iftikhar Jan.

In: Arteriosclerosis, thrombosis, and vascular biology, Vol. 39, No. 6, 01.06.2019, p. 1227-1233.

Research output: Contribution to journalArticle

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