TY - JOUR
T1 - Targeted metagenomics for clinical detection and discovery of bacterial tick-borne pathogens
AU - Kingry, Luke
AU - Sheldon, Sarah
AU - Oatman, Stephanie
AU - Pritt, Bobbi
AU - Anacker, Melissa
AU - Bjork, Jenna
AU - Neitzel, David
AU - Strain, Anna
AU - Berry, Jon
AU - Sloan, Lynne
AU - Respicio-Kingry, Laurel
AU - Dietrich, Elizabeth
AU - Bloch, Karen
AU - Moncayo, Abelardo
AU - Srinivasamoorthy, Ganesh
AU - Hu, Bin
AU - Hinckley, Alison
AU - Mead, Paul
AU - Kugeler, Kiersten
AU - Petersena, Jeannine
N1 - Publisher Copyright:
© 2020 American Society for Microbiology. All Rights Reserved.
PY - 2020/11
Y1 - 2020/11
N2 - Tick-borne diseases, due to a diversity of bacterial pathogens, represent a significant and increasing public health threat throughout the Northern Hemisphere. A high-throughput 16S V1-V2 rRNA gene-based metagenomics assay was developed and evaluated using >13,000 residual samples from patients suspected of having tick-borne illness and >1,000 controls. Taxonomic predictions for tick-borne bacteria were exceptionally accurate, as independently validated by secondary testing. Overall, 881 specimens were positive for bacterial tick-borne agents. Twelve tickborne bacterial species were detected, including two novel pathogens, representing a 100% increase in the number of tick-borne bacteria identified compared to what was possible by initial PCR testing. In three blood specimens, two tick-borne bacteria were simultaneously detected. Seven bacteria, not known to be tick transmitted, were also confirmed to be unique to samples from persons suspected of having tick-borne illness. These results indicate that 16S V1-V2 metagenomics can greatly simplify diagnosis and accelerate the discovery of bacterial tick-borne pathogens.
AB - Tick-borne diseases, due to a diversity of bacterial pathogens, represent a significant and increasing public health threat throughout the Northern Hemisphere. A high-throughput 16S V1-V2 rRNA gene-based metagenomics assay was developed and evaluated using >13,000 residual samples from patients suspected of having tick-borne illness and >1,000 controls. Taxonomic predictions for tick-borne bacteria were exceptionally accurate, as independently validated by secondary testing. Overall, 881 specimens were positive for bacterial tick-borne agents. Twelve tickborne bacterial species were detected, including two novel pathogens, representing a 100% increase in the number of tick-borne bacteria identified compared to what was possible by initial PCR testing. In three blood specimens, two tick-borne bacteria were simultaneously detected. Seven bacteria, not known to be tick transmitted, were also confirmed to be unique to samples from persons suspected of having tick-borne illness. These results indicate that 16S V1-V2 metagenomics can greatly simplify diagnosis and accelerate the discovery of bacterial tick-borne pathogens.
KW - Anaplasmosis
KW - Human granulocytic ehrlichiosis
KW - Lyme disease
KW - Metagenomics
KW - Tick-borne bacteria
KW - Vector-borne diseases
UR - http://www.scopus.com/inward/record.url?scp=85094220908&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85094220908&partnerID=8YFLogxK
U2 - 10.1128/JCM.00147-20
DO - 10.1128/JCM.00147-20
M3 - Article
C2 - 32878950
AN - SCOPUS:85094220908
SN - 0095-1137
VL - 58
JO - Journal of clinical microbiology
JF - Journal of clinical microbiology
IS - 11
M1 - e00147-20
ER -