@inbook{646b687f6989488fab17f3e0a5197721,
title = "TALEN-Mediated mutagenesis and genome editing",
abstract = "Transcription activator-like effectors (TALEs) are important genomic tools with customizable DNA-binding motifsfor locus-specific modifications. In particular, TALE nucleases or TALENs have been successfully used in the zebrafish model system to introduce targeted mutations via repair of double-stranded breaks (DSBs) either through nonhomologous end joining (NHEJ) or by homology-directed repair (HDR) and homology-independent repair in the presence of a donor template. Compared with other customizable nucleases, TALENs offer high binding specificity and fewer sequence constraints in targeting the genome, with comparable mutagenic activity. Here, we describe a detailed in silico design tool for zebrafish genome editing for TALENs and CRISPR/Cas9 custom restriction enzymes using Mojo Hand 2.0 software.",
keywords = "Customized nucleases, FusX, Genome editing, Golden Gate, TALEN, Zebrafish",
author = "Ma, {Alvin C.H.} and Yi Chen and Blackburn, {Patrick R.} and Ekker, {Stephen C.}",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2016.",
year = "2016",
doi = "10.1007/978-1-4939-3771-4_2",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "17--30",
booktitle = "Methods in Molecular Biology",
}