TALEN-Mediated mutagenesis and genome editing

Alvin C H Ma, Yi Chen, Patrick R. Blackburn, Stephen C Ekker

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Citations (Scopus)

Abstract

Transcription activator-like effectors (TALEs) are important genomic tools with customizable DNA-binding motifsfor locus-specific modifications. In particular, TALE nucleases or TALENs have been successfully used in the zebrafish model system to introduce targeted mutations via repair of double-stranded breaks (DSBs) either through nonhomologous end joining (NHEJ) or by homology-directed repair (HDR) and homology-independent repair in the presence of a donor template. Compared with other customizable nucleases, TALENs offer high binding specificity and fewer sequence constraints in targeting the genome, with comparable mutagenic activity. Here, we describe a detailed in silico design tool for zebrafish genome editing for TALENs and CRISPR/Cas9 custom restriction enzymes using Mojo Hand 2.0 software.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages17-30
Number of pages14
Volume1451
DOIs
StatePublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1451
ISSN (Print)10643745

Fingerprint

Mutagenesis
Zebrafish
Targeted Gene Repair
Clustered Regularly Interspaced Short Palindromic Repeats
Computer Simulation
Software
Hand
Genome
Gene Editing
Transcription Activator-Like Effector Nucleases
DNA
Enzymes

Keywords

  • Customized nucleases
  • FusX
  • Genome editing
  • Golden Gate
  • TALEN
  • Zebrafish

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Ma, A. C. H., Chen, Y., Blackburn, P. R., & Ekker, S. C. (2016). TALEN-Mediated mutagenesis and genome editing. In Methods in Molecular Biology (Vol. 1451, pp. 17-30). (Methods in Molecular Biology; Vol. 1451). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3771-4_2

TALEN-Mediated mutagenesis and genome editing. / Ma, Alvin C H; Chen, Yi; Blackburn, Patrick R.; Ekker, Stephen C.

Methods in Molecular Biology. Vol. 1451 Humana Press Inc., 2016. p. 17-30 (Methods in Molecular Biology; Vol. 1451).

Research output: Chapter in Book/Report/Conference proceedingChapter

Ma, ACH, Chen, Y, Blackburn, PR & Ekker, SC 2016, TALEN-Mediated mutagenesis and genome editing. in Methods in Molecular Biology. vol. 1451, Methods in Molecular Biology, vol. 1451, Humana Press Inc., pp. 17-30. https://doi.org/10.1007/978-1-4939-3771-4_2
Ma ACH, Chen Y, Blackburn PR, Ekker SC. TALEN-Mediated mutagenesis and genome editing. In Methods in Molecular Biology. Vol. 1451. Humana Press Inc. 2016. p. 17-30. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-3771-4_2
Ma, Alvin C H ; Chen, Yi ; Blackburn, Patrick R. ; Ekker, Stephen C. / TALEN-Mediated mutagenesis and genome editing. Methods in Molecular Biology. Vol. 1451 Humana Press Inc., 2016. pp. 17-30 (Methods in Molecular Biology).
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