TY - GEN
T1 - Tailoring fast-scan cyclic voltammetry for tonic dopamine concentration measurement
AU - Oh, Yoonbae
AU - Kang, Yu Min
AU - Park, Cheonho
AU - Shin, Hojin
AU - Kim, In Young
AU - Jang, Dong Pyo
AU - Bennet, Kevin E.
AU - Lee, Kendall H.
N1 - Publisher Copyright:
© 2017 IEEE.
PY - 2017/7/19
Y1 - 2017/7/19
N2 - Fast-scan cyclic voltammetry (FSCV) with background subtraction method has been widely used for detecting neurotransmitters in the brain. The most common application of FSCV is measuring phasic change of dopamine (DA) in the brain evoked by an external stimulus. The background subtraction method has greatly improved FSCV application to the neuroscience field, however, tonic dopamine concentration which is as vital as phasic change cannot be measured because the background is subtracted. In this study, we developed a tailoring FSCV technique which can manipulate background current by modifying the waveform voltage point. By using this technique, the last background current generated by last waveform in multiple pulses is tailored to the front background current. As a result, background current is cancelled out by subtracting the front voltammogram and tailored (last) voltammogram. On the other hand, DA oxidation/reduction pattern remained between front and last voltammogram, so that, tailoring FSCV can detect tonic DA concentration without background subtraction method. The tailoring technique is evaluated by comparing with commercialized enzyme-linked immunosorbent assay (ELISA) kits. By measuring endogenously released DA from DA cell, tailoring method showed significant correlation with ELISA result.
AB - Fast-scan cyclic voltammetry (FSCV) with background subtraction method has been widely used for detecting neurotransmitters in the brain. The most common application of FSCV is measuring phasic change of dopamine (DA) in the brain evoked by an external stimulus. The background subtraction method has greatly improved FSCV application to the neuroscience field, however, tonic dopamine concentration which is as vital as phasic change cannot be measured because the background is subtracted. In this study, we developed a tailoring FSCV technique which can manipulate background current by modifying the waveform voltage point. By using this technique, the last background current generated by last waveform in multiple pulses is tailored to the front background current. As a result, background current is cancelled out by subtracting the front voltammogram and tailored (last) voltammogram. On the other hand, DA oxidation/reduction pattern remained between front and last voltammogram, so that, tailoring FSCV can detect tonic DA concentration without background subtraction method. The tailoring technique is evaluated by comparing with commercialized enzyme-linked immunosorbent assay (ELISA) kits. By measuring endogenously released DA from DA cell, tailoring method showed significant correlation with ELISA result.
KW - Dopamine
KW - Fast-scan cyclic voltammetry
KW - Tonic DA concentration
UR - http://www.scopus.com/inward/record.url?scp=85027896089&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85027896089&partnerID=8YFLogxK
U2 - 10.1109/MeMeA.2017.7985858
DO - 10.1109/MeMeA.2017.7985858
M3 - Conference contribution
AN - SCOPUS:85027896089
T3 - 2017 IEEE International Symposium on Medical Measurements and Applications, MeMeA 2017 - Proceedings
SP - 106
EP - 110
BT - 2017 IEEE International Symposium on Medical Measurements and Applications, MeMeA 2017 - Proceedings
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 12th IEEE International Symposium on Medical Measurements and Applications, MeMeA 2017
Y2 - 7 May 2017 through 10 May 2017
ER -