T cell suppression as a mechanism for tolerance to MUC1 antigen in MUC1 transgenic mice

Dongshu Chen, Shigeo Koido, Yongqing Li, Sandra J Gendler, Jianlin Gong

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

C57BL/6 mice transgenic for human MUC1 (MUC1.Tg) have been developed to study immunologic responsiveness to the human MUC1 tumor-associated antigen. In the present studies, MUC1. Tg mice were immunized with purified human MUC1 antigen and irradiated MUC1-positive (MC38/MUC1) tumor cells. Immunization with MUC1 antigen was associated with induction of an anti-MUC1 antibody response and no detectable cytotoxic T cell reactivity. Similar findings were obtained after immunization with irradiated MC38/MUC1 tumor cells. The results also demonstrate that immunization of MUC1.Tg mice with MUC1 is associated with decreased levels of CD8+ T cells. In addition, expression of αβ T cell receptors and CD28 were down-regulated on CD8+ T cells as a consequence of MUC1 immunization. These findings support a role for T cell suppression in tolerance to MUC1 antigen in MUC1.Tg mice.

Original languageEnglish (US)
Pages (from-to)107-115
Number of pages9
JournalBreast Cancer Research and Treatment
Volume60
Issue number2
DOIs
StatePublished - 2000

Fingerprint

Transgenic Mice
Immunization
T-Lymphocytes
Antigens
Neoplasm Antigens
T-Cell Antigen Receptor
Inbred C57BL Mouse
Antibody Formation
Anti-Idiotypic Antibodies
Neoplasms

Keywords

  • Anti-tumor immunity
  • MUC1 antigen
  • T cell suppression
  • Tolerance
  • Transgenic mice

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

T cell suppression as a mechanism for tolerance to MUC1 antigen in MUC1 transgenic mice. / Chen, Dongshu; Koido, Shigeo; Li, Yongqing; Gendler, Sandra J; Gong, Jianlin.

In: Breast Cancer Research and Treatment, Vol. 60, No. 2, 2000, p. 107-115.

Research output: Contribution to journalArticle

Chen, Dongshu ; Koido, Shigeo ; Li, Yongqing ; Gendler, Sandra J ; Gong, Jianlin. / T cell suppression as a mechanism for tolerance to MUC1 antigen in MUC1 transgenic mice. In: Breast Cancer Research and Treatment. 2000 ; Vol. 60, No. 2. pp. 107-115.
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AU - Koido, Shigeo

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AU - Gendler, Sandra J

AU - Gong, Jianlin

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N2 - C57BL/6 mice transgenic for human MUC1 (MUC1.Tg) have been developed to study immunologic responsiveness to the human MUC1 tumor-associated antigen. In the present studies, MUC1. Tg mice were immunized with purified human MUC1 antigen and irradiated MUC1-positive (MC38/MUC1) tumor cells. Immunization with MUC1 antigen was associated with induction of an anti-MUC1 antibody response and no detectable cytotoxic T cell reactivity. Similar findings were obtained after immunization with irradiated MC38/MUC1 tumor cells. The results also demonstrate that immunization of MUC1.Tg mice with MUC1 is associated with decreased levels of CD8+ T cells. In addition, expression of αβ T cell receptors and CD28 were down-regulated on CD8+ T cells as a consequence of MUC1 immunization. These findings support a role for T cell suppression in tolerance to MUC1 antigen in MUC1.Tg mice.

AB - C57BL/6 mice transgenic for human MUC1 (MUC1.Tg) have been developed to study immunologic responsiveness to the human MUC1 tumor-associated antigen. In the present studies, MUC1. Tg mice were immunized with purified human MUC1 antigen and irradiated MUC1-positive (MC38/MUC1) tumor cells. Immunization with MUC1 antigen was associated with induction of an anti-MUC1 antibody response and no detectable cytotoxic T cell reactivity. Similar findings were obtained after immunization with irradiated MC38/MUC1 tumor cells. The results also demonstrate that immunization of MUC1.Tg mice with MUC1 is associated with decreased levels of CD8+ T cells. In addition, expression of αβ T cell receptors and CD28 were down-regulated on CD8+ T cells as a consequence of MUC1 immunization. These findings support a role for T cell suppression in tolerance to MUC1 antigen in MUC1.Tg mice.

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