TY - JOUR
T1 - T-cell heterogeneity in muscle lesions of inclusion body myositis
AU - Bender, Anke
AU - Behrens, Lüder
AU - Engel, Andrew G.
AU - Hohlfeld, Reinhard
N1 - Funding Information:
This study was supported by the Deutsche Forschungsgemeinschaft (SFB 217, C13), Wilhelm Sander Stiftung (94.068.1), Max Planck Society (R.H., A.B., and L.B.), and NS-6277 from NIH and a Research Grant from the MDA (A.G.E.).
PY - 1998/4/1
Y1 - 1998/4/1
N2 - In the muscle lesions of inclusion body myositis (IBM), two populations of T cells can morphologically be distinguished, T cells that invade muscle fibers and T cells that remain in interstitial areas. Using combined immunohistochemistry and RT-PCR, we analysed the TCR expressed by these distinct T cell populations. In three of eight IBM muscle specimens, the autoinvasive T cells were stained with one of the available mAbs: anti- Vβ5.3 in patient 1, anti-Vβ5.2 in patient 2, and anti-Vβ3 in patient 3. The corresponding TCR Vβ mRNAs were amplified by RT-PCR and the PCR products were cloned and sequenced. In all three specimens, the TCRs expressed by the autoinvasive T cells were clonally restricted. Furthermore, in patient 1 two different mAbs labelled two distinct populations of T cells: an autoaggressive population of CD8 + Vβ5.3 + cells that invaded muscle fibers and a noninvasive interstitial population of CD8 - Vβ5.1 + cells. TCR sequence analysis revealed that the noninvasive Vβ5.1 + T cells were clonally diverse, whereas the autoinvasive Vβ5.3 + T cells were clonally restricted.
AB - In the muscle lesions of inclusion body myositis (IBM), two populations of T cells can morphologically be distinguished, T cells that invade muscle fibers and T cells that remain in interstitial areas. Using combined immunohistochemistry and RT-PCR, we analysed the TCR expressed by these distinct T cell populations. In three of eight IBM muscle specimens, the autoinvasive T cells were stained with one of the available mAbs: anti- Vβ5.3 in patient 1, anti-Vβ5.2 in patient 2, and anti-Vβ3 in patient 3. The corresponding TCR Vβ mRNAs were amplified by RT-PCR and the PCR products were cloned and sequenced. In all three specimens, the TCRs expressed by the autoinvasive T cells were clonally restricted. Furthermore, in patient 1 two different mAbs labelled two distinct populations of T cells: an autoaggressive population of CD8 + Vβ5.3 + cells that invaded muscle fibers and a noninvasive interstitial population of CD8 - Vβ5.1 + cells. TCR sequence analysis revealed that the noninvasive Vβ5.1 + T cells were clonally diverse, whereas the autoinvasive Vβ5.3 + T cells were clonally restricted.
KW - Autoimmunity
KW - Inflammatory muscle disease
KW - TCR
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U2 - 10.1016/S0165-5728(97)00246-4
DO - 10.1016/S0165-5728(97)00246-4
M3 - Article
C2 - 9600712
AN - SCOPUS:0032054564
SN - 0165-5728
VL - 84
SP - 86
EP - 91
JO - Journal of neuroimmunology
JF - Journal of neuroimmunology
IS - 1
ER -