T-cell heterogeneity in muscle lesions of inclusion body myositis

Anke Bender, Lüder Behrens, Andrew G. Engel, Reinhard Hohlfeld

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

In the muscle lesions of inclusion body myositis (IBM), two populations of T cells can morphologically be distinguished, T cells that invade muscle fibers and T cells that remain in interstitial areas. Using combined immunohistochemistry and RT-PCR, we analysed the TCR expressed by these distinct T cell populations. In three of eight IBM muscle specimens, the autoinvasive T cells were stained with one of the available mAbs: anti- Vβ5.3 in patient 1, anti-Vβ5.2 in patient 2, and anti-Vβ3 in patient 3. The corresponding TCR Vβ mRNAs were amplified by RT-PCR and the PCR products were cloned and sequenced. In all three specimens, the TCRs expressed by the autoinvasive T cells were clonally restricted. Furthermore, in patient 1 two different mAbs labelled two distinct populations of T cells: an autoaggressive population of CD8 + Vβ5.3 + cells that invaded muscle fibers and a noninvasive interstitial population of CD8 - Vβ5.1 + cells. TCR sequence analysis revealed that the noninvasive Vβ5.1 + T cells were clonally diverse, whereas the autoinvasive Vβ5.3 + T cells were clonally restricted.

Original languageEnglish (US)
Pages (from-to)86-91
Number of pages6
JournalJournal of neuroimmunology
Volume84
Issue number1
DOIs
StatePublished - Apr 1 1998

Keywords

  • Autoimmunity
  • Inflammatory muscle disease
  • TCR

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

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