Abstract
The detection of peripheral blood plasma cells is clinically important, but difficult to perform by use of routine smears. To simplify the detection process, the peripheral blood T cells from ten patients with known active multiple myeloma were depleted using anti-CD2 coated magnetic beads. In all cases, there was enrichment of the immunoglobulin (Ig) positive cells after T cell depletion (mean enrichment factor, 3.4; median, 3.2; range, 1.2-5.1) with a mean pre-bead %Ig+ cells of 7.3 compared to 20.4 for the post-bead sample (p = 0.005). The monoclonal plasma cells were similarly enriched and more easily enumerated on the microscope slides prepared from the T cell depleted sample.
Original language | English (US) |
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Pages (from-to) | 253-256 |
Number of pages | 4 |
Journal | Journal of Immunological Methods |
Volume | 144 |
Issue number | 2 |
DOIs | |
State | Published - Nov 22 1991 |
Keywords
- Magnetic beads
- Multiple myeloma
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology