Systems approach to neurodegenerative disease biomarker discovery

Christopher Lausted, Inyoul Lee, Yong Zhou, Shizhen Qin, Jaeyun Sung, Nathan D. Price, Leroy Hood, Kai Wang

Research output: Contribution to journalReview article

31 Scopus citations

Abstract

Biomarkers are essential for performing early diagnosis, monitoring neurodegenerative disease progression, gauging responses to therapies, and stratifying neurodegenerative diseases into their different subtypes. A wide range of molecular markers are under investigation in tissues and biofluids as well as through imaging; moreover, many are prominent proteins present in cerebrospinal fluid. However, in more frequently and easily collected fluids such as plasma, these proteins show only a modest correlation with disease and thus lack the necessary sensitivity or specificity for clinical use. High-throughput and quantitative proteomic technologies and systems-driven approaches to biofluid analysis are now being utilized in the search for better biomarkers. Biomarker discovery involves many critical steps including study design, sample preparation, protein and peptide separation and identification, and bioinformatics and data integration issues that must be carefully controlled before independent confirmation and validation. In this review, we summarize current proteomic and nucleic acid technologies involved in the discovery of biomarkers of neurodegenerative diseases, particularly Alzheimer's, Parkinson's, Huntington's, and prion diseases.

Original languageEnglish (US)
Pages (from-to)457-481
Number of pages25
JournalAnnual Review of Pharmacology and Toxicology
Volume54
DOIs
StatePublished - Jan 2014

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Keywords

  • Animal models
  • MicroRNA
  • Next-generation sequencing
  • Proteomics
  • Selected reaction monitoring
  • Systems biology

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology

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