Systemic free fatty acid disposal into very low-Density lipoprotein triglycerides

Christina Koutsari, Manpreet S. Mundi, Asem H. Ali, Bruce W. Patterson, Michael Dennis Jensen

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We measured the incorporation of systemic free fatty acids (FFA) into circulating very low-density lipoprotein triglycerides (VLDLTGs) under postabsorptive, postprandial, and walking conditions in humans. Fifty-five men and 85 premenopausal women with BMI 18-24 (lean) and 27-36 kg/m2 (overweight/obese) received an intravenous bolus injection of [1,1,2,3,3- 2H5]glycerol (to measure VLDL-TG kinetics) and either [1-14C]palmitate or [9,10-3H] palmitate to determine the proportion of systemic FFA that is converted to VLDL-TG. Experiments started at 0630 h after a 12-h overnight fast. In the postabsorptive protocol, participants rested and remained fasted until 1330 h. In the postprandial protocol, volunteers ingested frequent portions of a fat-free smoothie. In the walking protocol, participants walked on a treadmillfor 5.5 h at ̃ 33 resting energy expenditure. Approximately 7% of circulating FFA was converted into VLDL-TG. VLDL-TG secretion rates (SRs) were not statistically different among protocols. Visceral fat mass was the only independent predictor of VLDL-TG secretion, explaining 33-57% of the variance. The small proportion of systemic FFA that is converted to VLDL-TG can confound the expected relationship between plasma FFA concentration and VLDL-TG SRs. Regulation of VLDL-TG secretion is complex in that, despite a broad spectrum of physiological FFA concentrations, VLDL-TG SRs did not vary based on different acute substrate availability.

Original languageEnglish (US)
Pages (from-to)2386-2395
Number of pages10
JournalDiabetes
Volume62
Issue number7
DOIs
StatePublished - Jul 2013

Fingerprint

Nonesterified Fatty Acids
Palmitates
Walking
Intra-Abdominal Fat
Intravenous Injections
Glycerol
Energy Metabolism
very low density lipoprotein triglyceride
Volunteers
Fats

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Systemic free fatty acid disposal into very low-Density lipoprotein triglycerides. / Koutsari, Christina; Mundi, Manpreet S.; Ali, Asem H.; Patterson, Bruce W.; Jensen, Michael Dennis.

In: Diabetes, Vol. 62, No. 7, 07.2013, p. 2386-2395.

Research output: Contribution to journalArticle

Koutsari, C, Mundi, MS, Ali, AH, Patterson, BW & Jensen, MD 2013, 'Systemic free fatty acid disposal into very low-Density lipoprotein triglycerides', Diabetes, vol. 62, no. 7, pp. 2386-2395. https://doi.org/10.2337/db12-1557
Koutsari, Christina ; Mundi, Manpreet S. ; Ali, Asem H. ; Patterson, Bruce W. ; Jensen, Michael Dennis. / Systemic free fatty acid disposal into very low-Density lipoprotein triglycerides. In: Diabetes. 2013 ; Vol. 62, No. 7. pp. 2386-2395.
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