System-wide associations between DNA-methylation, gene expression, and humoral immune response to influenza vaccination

Michael T. Zimmermann, Ann L Oberg, Diane E. Grill, Inna G. Ovsyannikova, Iana H. Haralambieva, Richard B Kennedy, Gregory A. Poland

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Failure to achieve a protected state after influenza vaccination is poorly understood but occurs commonly among aged populations experiencing greater immunosenescence. In order to better understand immune response in the elderly, we studied epigenetic and transcriptomic profiles and humoral immune response outcomes in 50-74 year old healthy participants. Associations between DNA methylation and gene expression reveal a systemwide regulation of immune-relevant functions, likely playing a role in regulating a participant's propensity to respond to vaccination. Our findings show that sites of methylation regulation associated with humoral response to vaccination impact known cellular differentiation signaling and antigen presentation pathways. We performed our analysis using per-site and regionally average methylation levels, in addition to continuous or dichotomized outcome measures. The genes and molecular functions implicated by each analysis were compared, highlighting different aspects of the biologic mechanisms of immune response affected by differential methylation. Both cis-acting (within the gene or promoter) and trans-acting (enhancers and transcription factor binding sites) sites show significant associations with measures of humoral immunity. Specifically, we identified a group of CpGs that, when coordinately hypo-methylated, are associated with lower humoral immune response, and methylated with higher response. Additionally, CpGs that individually predict humoral immune responses are enriched for polycomb-group and FOXP2 transcription factor binding sites. The most robust associations implicate differential methylation affecting gene expression levels of genes with known roles in immunity (e.g. HLA-B and HLA-DQB2) and immunosenescence. We believe our data and analysis strategy highlight new and interesting epigenetic trends affecting humoral response to vaccination against influenza; one of the most common and impactful viral pathogens.

Original languageEnglish (US)
Article numbere0152034
JournalPLoS One
Volume11
Issue number3
DOIs
StatePublished - Mar 31 2016

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Methylation
DNA methylation
DNA Methylation
Humoral Immunity
Gene expression
humoral immunity
influenza
Human Influenza
Vaccination
vaccination
Gene Expression
methylation
gene expression
Genes
Epigenomics
Transcription Factors
Binding Sites
epigenetics
Trans-Activators
HLA-B Antigens

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

System-wide associations between DNA-methylation, gene expression, and humoral immune response to influenza vaccination. / Zimmermann, Michael T.; Oberg, Ann L; Grill, Diane E.; Ovsyannikova, Inna G.; Haralambieva, Iana H.; Kennedy, Richard B; Poland, Gregory A.

In: PLoS One, Vol. 11, No. 3, e0152034, 31.03.2016.

Research output: Contribution to journalArticle

Zimmermann, Michael T. ; Oberg, Ann L ; Grill, Diane E. ; Ovsyannikova, Inna G. ; Haralambieva, Iana H. ; Kennedy, Richard B ; Poland, Gregory A. / System-wide associations between DNA-methylation, gene expression, and humoral immune response to influenza vaccination. In: PLoS One. 2016 ; Vol. 11, No. 3.
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