Synthetic α-subunit peptides stimulate testosterone production in vitro by rat leydig cells

Lisa D. Erickson, Stacey Rizza, Elizabeth R. Bergert, M. Cristine Charlesworth, Daniel J Mc Cormick, Robert J. Ryan

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The glycoprotein hormones (LH, hCG, FSH, and TSH) have a common 92-amino acid α-subunit which is noncovalently linked to a hormone-specific β-subunit. Synthetic peptides of the α-subunit have been shown to inhibit binding of [125I]iodo-hCG to rat ovarian membrane and [125I]iodo-TSH to human thyroid membrane preparations. Synthetic overlapping peptides of the α-subunit of hCG were prepared by solid phase techniques and tested in a standard in vitro rat Leydig cell bioassay. Three regions in the α-subunit (α1-15, α30-45, and α71-85) were found to stimulate testosterone production. All three regions correlate with inhibition of hCG binding to ovarian receptors, but subtle differences exist between the binding sites and effector sites. These data indicate that the glycoprotein α-subunit has intrinsic bioactivity.

Original languageEnglish (US)
Pages (from-to)2555-2560
Number of pages6
JournalEndocrinology
Volume126
Issue number5
StatePublished - May 1990

Fingerprint

Leydig Cells
Testosterone
Glycoproteins
Hormones
Peptides
Membranes
Biological Assay
Thyroid Gland
Binding Sites
Amino Acids
In Vitro Techniques
iodo-chorionic gonadotropin

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Erickson, L. D., Rizza, S., Bergert, E. R., Charlesworth, M. C., Mc Cormick, D. J., & Ryan, R. J. (1990). Synthetic α-subunit peptides stimulate testosterone production in vitro by rat leydig cells. Endocrinology, 126(5), 2555-2560.

Synthetic α-subunit peptides stimulate testosterone production in vitro by rat leydig cells. / Erickson, Lisa D.; Rizza, Stacey; Bergert, Elizabeth R.; Charlesworth, M. Cristine; Mc Cormick, Daniel J; Ryan, Robert J.

In: Endocrinology, Vol. 126, No. 5, 05.1990, p. 2555-2560.

Research output: Contribution to journalArticle

Erickson, LD, Rizza, S, Bergert, ER, Charlesworth, MC, Mc Cormick, DJ & Ryan, RJ 1990, 'Synthetic α-subunit peptides stimulate testosterone production in vitro by rat leydig cells', Endocrinology, vol. 126, no. 5, pp. 2555-2560.
Erickson, Lisa D. ; Rizza, Stacey ; Bergert, Elizabeth R. ; Charlesworth, M. Cristine ; Mc Cormick, Daniel J ; Ryan, Robert J. / Synthetic α-subunit peptides stimulate testosterone production in vitro by rat leydig cells. In: Endocrinology. 1990 ; Vol. 126, No. 5. pp. 2555-2560.
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