TY - JOUR
T1 - Synthetic α-subunit peptides stimulate testosterone production in vitro by rat leydig cells
AU - Erickson, Lisa D.
AU - Rizza, Stacey A.
AU - Bergert, Elizabeth R.
AU - Charlesworth, M. Cristine
AU - McCormick, Daniel J.
AU - Ryan, Robert J.
PY - 1990/5
Y1 - 1990/5
N2 - The glycoprotein hormones (LH, hCG, FSH, and TSH) have a common 92-amino acid α-subunit which is noncovalently linked to a hormone-specific β-subunit. Synthetic peptides of the α-subunit have been shown to inhibit binding of [125I]iodo-hCG to rat ovarian membrane and [125I]iodo-TSH to human thyroid membrane preparations. Synthetic overlapping peptides of the α-subunit of hCG were prepared by solid phase techniques and tested in a standard in vitro rat Leydig cell bioassay. Three regions in the α-subunit (α1-15, α30-45, and α71-85) were found to stimulate testosterone production. All three regions correlate with inhibition of hCG binding to ovarian receptors, but subtle differences exist between the binding sites and effector sites. These data indicate that the glycoprotein α-subunit has intrinsic bioactivity.
AB - The glycoprotein hormones (LH, hCG, FSH, and TSH) have a common 92-amino acid α-subunit which is noncovalently linked to a hormone-specific β-subunit. Synthetic peptides of the α-subunit have been shown to inhibit binding of [125I]iodo-hCG to rat ovarian membrane and [125I]iodo-TSH to human thyroid membrane preparations. Synthetic overlapping peptides of the α-subunit of hCG were prepared by solid phase techniques and tested in a standard in vitro rat Leydig cell bioassay. Three regions in the α-subunit (α1-15, α30-45, and α71-85) were found to stimulate testosterone production. All three regions correlate with inhibition of hCG binding to ovarian receptors, but subtle differences exist between the binding sites and effector sites. These data indicate that the glycoprotein α-subunit has intrinsic bioactivity.
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M3 - Article
C2 - 1691699
AN - SCOPUS:0025341089
SN - 0013-7227
VL - 126
SP - 2555
EP - 2560
JO - Endocrinology
JF - Endocrinology
IS - 5
ER -