Synthesis of a peptide-universal nucleotide antigen

Towards next-generation antibodies to detect topoisomerase I-DNA covalent complexes

Angela L. Perkins, Kevin L. Peterson, Thomas G. Beito, Karen S. Flatten, Scott H Kaufmann, Daniel A. Harki

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The topoisomerase (topo) I-DNA covalent complex represents an attractive target for developing diagnostic antibodies to measure responsiveness to drugs. We report a new antigen, peptide 2, and four murine monoclonal antibodies raised against 2 that exhibit excellent specificity for recognition of 2 in comparison to structurally similar peptides by enzyme-linked immunosorbent assays. Although topo I-DNA complex detection was not achieved in cellular samples by these new antibodies, a new strategy for antigen design is reported.

Original languageEnglish (US)
Pages (from-to)4103-4109
Number of pages7
JournalOrganic and Biomolecular Chemistry
Volume14
Issue number17
DOIs
StatePublished - 2016

Fingerprint

Type I DNA Topoisomerase
nucleotides
antigens
antibodies
peptides
Nucleotides
deoxyribonucleic acid
Antigens
Immunosorbents
Peptides
Antibodies
synthesis
Assays
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies
enzymes
drugs
Enzymes
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry
  • Organic Chemistry

Cite this

Synthesis of a peptide-universal nucleotide antigen : Towards next-generation antibodies to detect topoisomerase I-DNA covalent complexes. / Perkins, Angela L.; Peterson, Kevin L.; Beito, Thomas G.; Flatten, Karen S.; Kaufmann, Scott H; Harki, Daniel A.

In: Organic and Biomolecular Chemistry, Vol. 14, No. 17, 2016, p. 4103-4109.

Research output: Contribution to journalArticle

Perkins, Angela L. ; Peterson, Kevin L. ; Beito, Thomas G. ; Flatten, Karen S. ; Kaufmann, Scott H ; Harki, Daniel A. / Synthesis of a peptide-universal nucleotide antigen : Towards next-generation antibodies to detect topoisomerase I-DNA covalent complexes. In: Organic and Biomolecular Chemistry. 2016 ; Vol. 14, No. 17. pp. 4103-4109.
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