Synthesis and evaluation of near-infrared (NIR) dye-herceptin conjugates as photoacoustic computed tomography (PCT) probes for HER2 expression in breast cancer

Sibaprasad Bhattacharyya, Shuyan Wang, Daniel Reinecke, William Kiser, Robert A. Kruger, Timothy R DeGrado

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

We are evaluating PCT imaging in conjunction with NIR dye labeled Herceptin antibody for noninvasive assessment of HER2 expression in tumors. Herceptin was labeled with Alexa Fluor-750 amine reactive dye for characterization of photoacoustic and fluorescence signals. Measurements were performed in solution and after incubation in cultured cell lines that were positive or negative in expression of HER2. The dye to antibody ratio was controlled to achieve a broad range of degree of labeling (DOL = 2 to 15). Photoacoustic signal intensity of Herceptin-dye conjugates in solution increased with increases over the entire DOL range studied. In contrast, fluorescence exhibited significant quenching for higher DOL. In vitro PCT imaging of the labeled HER2 (+) and HER2 (-) cells revealed the targeting specificity of the NIR dye labeled Herceptin. In HER2 (+) cells lines, photoacoustic signal intensity gradually increased with increasing DOL and with increasing number of cells. These results demonstrate that PCT-based measurement of HER2 receptor binding using NIR dye labeled Herceptin is feasible. The absence of a quenching effect with increased DOL advantages this method over traditional methods based on fluorescence measurement.

Original languageEnglish (US)
Pages (from-to)1186-1193
Number of pages8
JournalBioconjugate Chemistry
Volume19
Issue number6
DOIs
StatePublished - Jun 2008
Externally publishedYes

Fingerprint

Photoacoustic effect
Tomography
Coloring Agents
Dyes
Breast Neoplasms
Infrared radiation
Fluorescence
Antibodies
Quenching
Cells
Imaging techniques
Cell Line
Labeling
Amines
Trastuzumab
Tumors
Cultured Cells
Cell Count
Neoplasms

ASJC Scopus subject areas

  • Chemistry(all)
  • Organic Chemistry
  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Synthesis and evaluation of near-infrared (NIR) dye-herceptin conjugates as photoacoustic computed tomography (PCT) probes for HER2 expression in breast cancer. / Bhattacharyya, Sibaprasad; Wang, Shuyan; Reinecke, Daniel; Kiser, William; Kruger, Robert A.; DeGrado, Timothy R.

In: Bioconjugate Chemistry, Vol. 19, No. 6, 06.2008, p. 1186-1193.

Research output: Contribution to journalArticle

Bhattacharyya, Sibaprasad ; Wang, Shuyan ; Reinecke, Daniel ; Kiser, William ; Kruger, Robert A. ; DeGrado, Timothy R. / Synthesis and evaluation of near-infrared (NIR) dye-herceptin conjugates as photoacoustic computed tomography (PCT) probes for HER2 expression in breast cancer. In: Bioconjugate Chemistry. 2008 ; Vol. 19, No. 6. pp. 1186-1193.
@article{63942c7225214e5a8e63eef213649595,
title = "Synthesis and evaluation of near-infrared (NIR) dye-herceptin conjugates as photoacoustic computed tomography (PCT) probes for HER2 expression in breast cancer",
abstract = "We are evaluating PCT imaging in conjunction with NIR dye labeled Herceptin antibody for noninvasive assessment of HER2 expression in tumors. Herceptin was labeled with Alexa Fluor-750 amine reactive dye for characterization of photoacoustic and fluorescence signals. Measurements were performed in solution and after incubation in cultured cell lines that were positive or negative in expression of HER2. The dye to antibody ratio was controlled to achieve a broad range of degree of labeling (DOL = 2 to 15). Photoacoustic signal intensity of Herceptin-dye conjugates in solution increased with increases over the entire DOL range studied. In contrast, fluorescence exhibited significant quenching for higher DOL. In vitro PCT imaging of the labeled HER2 (+) and HER2 (-) cells revealed the targeting specificity of the NIR dye labeled Herceptin. In HER2 (+) cells lines, photoacoustic signal intensity gradually increased with increasing DOL and with increasing number of cells. These results demonstrate that PCT-based measurement of HER2 receptor binding using NIR dye labeled Herceptin is feasible. The absence of a quenching effect with increased DOL advantages this method over traditional methods based on fluorescence measurement.",
author = "Sibaprasad Bhattacharyya and Shuyan Wang and Daniel Reinecke and William Kiser and Kruger, {Robert A.} and DeGrado, {Timothy R}",
year = "2008",
month = "6",
doi = "10.1021/bc700482u",
language = "English (US)",
volume = "19",
pages = "1186--1193",
journal = "Bioconjugate Chemistry",
issn = "1043-1802",
publisher = "American Chemical Society",
number = "6",

}

TY - JOUR

T1 - Synthesis and evaluation of near-infrared (NIR) dye-herceptin conjugates as photoacoustic computed tomography (PCT) probes for HER2 expression in breast cancer

AU - Bhattacharyya, Sibaprasad

AU - Wang, Shuyan

AU - Reinecke, Daniel

AU - Kiser, William

AU - Kruger, Robert A.

AU - DeGrado, Timothy R

PY - 2008/6

Y1 - 2008/6

N2 - We are evaluating PCT imaging in conjunction with NIR dye labeled Herceptin antibody for noninvasive assessment of HER2 expression in tumors. Herceptin was labeled with Alexa Fluor-750 amine reactive dye for characterization of photoacoustic and fluorescence signals. Measurements were performed in solution and after incubation in cultured cell lines that were positive or negative in expression of HER2. The dye to antibody ratio was controlled to achieve a broad range of degree of labeling (DOL = 2 to 15). Photoacoustic signal intensity of Herceptin-dye conjugates in solution increased with increases over the entire DOL range studied. In contrast, fluorescence exhibited significant quenching for higher DOL. In vitro PCT imaging of the labeled HER2 (+) and HER2 (-) cells revealed the targeting specificity of the NIR dye labeled Herceptin. In HER2 (+) cells lines, photoacoustic signal intensity gradually increased with increasing DOL and with increasing number of cells. These results demonstrate that PCT-based measurement of HER2 receptor binding using NIR dye labeled Herceptin is feasible. The absence of a quenching effect with increased DOL advantages this method over traditional methods based on fluorescence measurement.

AB - We are evaluating PCT imaging in conjunction with NIR dye labeled Herceptin antibody for noninvasive assessment of HER2 expression in tumors. Herceptin was labeled with Alexa Fluor-750 amine reactive dye for characterization of photoacoustic and fluorescence signals. Measurements were performed in solution and after incubation in cultured cell lines that were positive or negative in expression of HER2. The dye to antibody ratio was controlled to achieve a broad range of degree of labeling (DOL = 2 to 15). Photoacoustic signal intensity of Herceptin-dye conjugates in solution increased with increases over the entire DOL range studied. In contrast, fluorescence exhibited significant quenching for higher DOL. In vitro PCT imaging of the labeled HER2 (+) and HER2 (-) cells revealed the targeting specificity of the NIR dye labeled Herceptin. In HER2 (+) cells lines, photoacoustic signal intensity gradually increased with increasing DOL and with increasing number of cells. These results demonstrate that PCT-based measurement of HER2 receptor binding using NIR dye labeled Herceptin is feasible. The absence of a quenching effect with increased DOL advantages this method over traditional methods based on fluorescence measurement.

UR - http://www.scopus.com/inward/record.url?scp=45749090194&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=45749090194&partnerID=8YFLogxK

U2 - 10.1021/bc700482u

DO - 10.1021/bc700482u

M3 - Article

C2 - 18505279

AN - SCOPUS:45749090194

VL - 19

SP - 1186

EP - 1193

JO - Bioconjugate Chemistry

JF - Bioconjugate Chemistry

SN - 1043-1802

IS - 6

ER -