Synovial protein kinase C and its apparent insensitivity to interleukin-1

K. I. Hulkower, R. Sagi-Eisenberg, L. M. Traub, H. I. Georgescu, Christopher H Evans

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Lapine synovial fibroblasts produce prostaglandin E2 (PGE2) and neutral metalloproteinases in response to phorbol 12-myristate 13-acetate (PMA), human recombinant interleukin-1 (hrIL-1) and, in an autocrine fashion, in response to partially purified preparations of their own cytokines known as cell-activating factors (CAF). Here we have examined the possible role of protein kinase C (PKC) in these responses. Whereas the 80-kDa substrate for PKC could not be detected in synovial fibroblasts, these cells contained a 35-kDa protein which fulfilled the criteria for qualifying as a specific substrate of PKC. Translocation assays based upon phosphorylation of the 35-kDa protein and Western blotting techniques allowed the movement of PKC from the cytosolic to the particulate fraction in response to PMA and CAF to be detected but not in response to 4α-PMA or hrIL-1. Inhibitors of PKC suppressed synovial activation by PMA, partially blocked activation by CAF but had no effect on activation by hrIL-1. There thus appear to be PKC-dependent and PKC-independent routes to synovial cell activation. Our data suggest that IL-1 uses the latter, while CAF contains cytokines which utilize both routes.

Original languageEnglish (US)
Pages (from-to)81-88
Number of pages8
JournalEuropean Journal of Biochemistry
Volume209
Issue number1
DOIs
StatePublished - 1992
Externally publishedYes

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Interleukin-1
Protein Kinase C
Acetates
Chemical activation
Fibroblasts
Cytokines
Metalloproteases
Phosphorylation
Dinoprostone
Substrates
Proteins
Western Blotting
Assays
phorbol-12-myristate

ASJC Scopus subject areas

  • Biochemistry

Cite this

Synovial protein kinase C and its apparent insensitivity to interleukin-1. / Hulkower, K. I.; Sagi-Eisenberg, R.; Traub, L. M.; Georgescu, H. I.; Evans, Christopher H.

In: European Journal of Biochemistry, Vol. 209, No. 1, 1992, p. 81-88.

Research output: Contribution to journalArticle

Hulkower, K. I. ; Sagi-Eisenberg, R. ; Traub, L. M. ; Georgescu, H. I. ; Evans, Christopher H. / Synovial protein kinase C and its apparent insensitivity to interleukin-1. In: European Journal of Biochemistry. 1992 ; Vol. 209, No. 1. pp. 81-88.
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