Synectin/syndecan-4 regulate coronary arteriolar growth during development

Eduard I. Dedkov, Mathew T. Thomas, Milan Sonka, Fuxing Yang, Thomas W. Chittenden, John M. Rhodes, Michael Simons, Erik L. Ritman, Robert J. Tomanek

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Syndecan-4 and its cytoplasmic binding partner, synectin, are known to play a role in FGF-2 signaling and vascular growth. To determine their roles in coronary artery/arteriolar formation and growth, we compared syndecan-4 and synectin null mice with their wild-type counterparts. Image analysis of arterioles visualized by smooth muscle α-actin immunostaining revealed that synectin -/- mice had lower arteriolar length and volume densities than wild-type mice. As shown by electron microscopic analysis, arterioles from the two did not differ in morphology, including their endothelial cell junctions, and the organization and distribution of smooth muscle. Using micro-computer tomography, we found that the size and branching patterns of coronary arteries (diameters > 50 μm) were similar for the two groups, a finding that indicates that the growth of arteries is not influenced by a loss of synectin. Syndecan-4 null male mice also had lower arteriolar length densities than their gender wild-type controls. However, female syndecan-4 null mice were characterized by higher arteriolar length and volume densities than their gender-matched wild-type controls. Thus, we conclude that both synectin and syndecan-4 play a role in arteriolar development, a finding that is consistent with previous evidence that FGF-2 plays a role in coronary arterial growth. Moreover, our data reveal that gender influences the arteriolar growth response to syndecan-4 but not to synectin.

Original languageEnglish (US)
Pages (from-to)2004-2010
Number of pages7
JournalDevelopmental Dynamics
Volume236
Issue number7
DOIs
StatePublished - Jul 2007

Fingerprint

Syndecan-4
Growth and Development
Growth
Arterioles
Fibroblast Growth Factor 2
Smooth Muscle
Coronary Vessels
Intercellular Junctions
Blood Vessels
Actins
Endothelial Cells
Arteries
Tomography
Electrons

Keywords

  • Arteriogenesis
  • Coronary arteriolar growth
  • Mice
  • Synectin/syndecan-4

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Dedkov, E. I., Thomas, M. T., Sonka, M., Yang, F., Chittenden, T. W., Rhodes, J. M., ... Tomanek, R. J. (2007). Synectin/syndecan-4 regulate coronary arteriolar growth during development. Developmental Dynamics, 236(7), 2004-2010. https://doi.org/10.1002/dvdy.21201

Synectin/syndecan-4 regulate coronary arteriolar growth during development. / Dedkov, Eduard I.; Thomas, Mathew T.; Sonka, Milan; Yang, Fuxing; Chittenden, Thomas W.; Rhodes, John M.; Simons, Michael; Ritman, Erik L.; Tomanek, Robert J.

In: Developmental Dynamics, Vol. 236, No. 7, 07.2007, p. 2004-2010.

Research output: Contribution to journalArticle

Dedkov, EI, Thomas, MT, Sonka, M, Yang, F, Chittenden, TW, Rhodes, JM, Simons, M, Ritman, EL & Tomanek, RJ 2007, 'Synectin/syndecan-4 regulate coronary arteriolar growth during development', Developmental Dynamics, vol. 236, no. 7, pp. 2004-2010. https://doi.org/10.1002/dvdy.21201
Dedkov EI, Thomas MT, Sonka M, Yang F, Chittenden TW, Rhodes JM et al. Synectin/syndecan-4 regulate coronary arteriolar growth during development. Developmental Dynamics. 2007 Jul;236(7):2004-2010. https://doi.org/10.1002/dvdy.21201
Dedkov, Eduard I. ; Thomas, Mathew T. ; Sonka, Milan ; Yang, Fuxing ; Chittenden, Thomas W. ; Rhodes, John M. ; Simons, Michael ; Ritman, Erik L. ; Tomanek, Robert J. / Synectin/syndecan-4 regulate coronary arteriolar growth during development. In: Developmental Dynamics. 2007 ; Vol. 236, No. 7. pp. 2004-2010.
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