Syncytia formation affects the yield and cytotoxicity of an adenovirus expressing a fusogenic glycoprotein at a late stage of replication

S. Guedan, A. Gros, M. Cascallo, Richard Geoffrey Vile, E. Mercade, R. Alemany

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Fusogenic membrane glycoproteins (FMGs) may enhance the cytotoxicity of conditionally replicative adenoviruses. However, expression at early stages of infection impairs virus replication. We have inserted the hyperfusogenic form of the gibbon ape leukemia virus (GALV) envelope glycoprotein as a new splice unit of the major late promoter (MLP) to generate a replication-competent adenovirus expressing this protein. At high multiplicity of infection (MOI), this virus replicated efficiently forming clumps of fused cells and showing a faster release. In contrast, at low MOI, infected cells formed syncytia where only one nucleus contained virus DNA, decreasing total virus production but increasing cytotoxicity.

Original languageEnglish (US)
Pages (from-to)1240-1245
Number of pages6
JournalGene Therapy
Volume15
Issue number17
DOIs
StatePublished - 2008

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Giant Cells
Adenoviridae
Glycoproteins
Gibbon ape leukemia virus
DNA Viruses
Membrane Glycoproteins
Virus Diseases
Virus Replication
Infection
Viruses
Proteins

ASJC Scopus subject areas

  • Genetics

Cite this

Syncytia formation affects the yield and cytotoxicity of an adenovirus expressing a fusogenic glycoprotein at a late stage of replication. / Guedan, S.; Gros, A.; Cascallo, M.; Vile, Richard Geoffrey; Mercade, E.; Alemany, R.

In: Gene Therapy, Vol. 15, No. 17, 2008, p. 1240-1245.

Research output: Contribution to journalArticle

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