Sustained apoptosis in human cardiac allografts despite histologic resolution of rejection

Sofia C. Masri, Mohamad H. Yamani, Mary A. Russell, Norman B. Ratliff, Jiacheng Yang, Alex Almasan, Carolyn Apperson-hansen, Jianbo Li, Randall C. Starling, Patrick McCarthy, James B. Young, Meredith Bond

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Background. We investigated the occurrence of apoptosis during and after resolution of cardiac allograft rejection. Apoptosis could play different roles in graft survival depending on the target cells; thus, we also determined the cell types involved. Methods. Endomyocardial biopsy specimens were evaluated during the first 6 months after transplantation as follows: group I, no current or prior rejection; group II, during an episode of moderate rejection; and group III, histologic resolution after an episode of moderate rejection. Results. Groups II and III showed significantly increased apoptotic activity, indicated by increased caspase-8 and caspase-3 activity; however, activated caspase-3 was undetectable in group I. Activated caspase-3 was detected only in groups II and III. Terminal deoxynucleotide transferase-mediated dUTP nick-end labeling was detected in groups II and III but not group I and predominantly in inflammatory cells. Conclusions. Increased caspase activity and apoptosis of infiltrating cells not only occurs during acute cardiac allograft rejection but persists after histologic resolution. Thus, programmed cell death occurs beyond the period of histologic resolution and may play a role in regulation of the rejection process.

Original languageEnglish (US)
Pages (from-to)859-864
Number of pages6
JournalTransplantation
Volume76
Issue number5
DOIs
StatePublished - Sep 15 2003

ASJC Scopus subject areas

  • Transplantation

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