Survey of urine from transplant recipients for polyomaviruses JC and BK using the polymerase chain reaction

W. F. Marshall, A. Telenti, J. Proper, Allen Jr. Aksamit, T. F. Smith

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Using polymerase chain reaction (PCR), we examined 108 urine specimens from 39 post transplant patients for polyomaviruses JC (JCV) and BK (BKV). Urine sediments were collected and subjected to 30 cycles of amplification. PCR products were resolved by agarose gel electrophoresis, transferred to nylon membranes by Southern blot, and hybridized with radiolabelled probes. Polyomavirus DNA was found in urine specimens from 17 out of 39 patients (44%). Both viruses were detected in specimens from nine patients, JCV alone in five, and BKV alone in three. In comparison, polyomavirus was detected in only five of 22 PCR positive specimens by shell vial cell culture assay. Our results show a high prevalence of polyomavirus shedding after transplantation and suggest a higher rate of JC viruria than previously reported.

Original languageEnglish (US)
Pages (from-to)125-128
Number of pages4
JournalMolecular and Cellular Probes
Volume5
Issue number2
DOIs
StatePublished - Jan 1 1991

Fingerprint

BK Virus
JC Virus
Polyomavirus
Urine
Polymerase Chain Reaction
Agar Gel Electrophoresis
Nylons
Southern Blotting
Cell Culture Techniques
Transplantation
Viruses
Transplants
Membranes
DNA
Transplant Recipients
Surveys and Questionnaires

Keywords

  • BK virus
  • JC virus
  • polymerase chain reaction
  • polyomavirus
  • transplantation

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Survey of urine from transplant recipients for polyomaviruses JC and BK using the polymerase chain reaction. / Marshall, W. F.; Telenti, A.; Proper, J.; Aksamit, Allen Jr.; Smith, T. F.

In: Molecular and Cellular Probes, Vol. 5, No. 2, 01.01.1991, p. 125-128.

Research output: Contribution to journalArticle

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