Surfactant protein D interacts with Pneumocystis carinii and mediates organism adherence to alveolar macrophages

Deirdre M. O'Riordan, Joseph E. Standing, Kun Young Kwon, Donald Chang, Edmond C. Crouch, Andrew Harold Limper

Research output: Contribution to journalArticle

141 Citations (Scopus)

Abstract

Pneumocystis carinii interacts with glycoproteins present in the lower respiratory tract through its mannose-rich surface antigen complex termed gpA. Surfactant protein D (SP-D) is a recently described component of the airspace lining material that possesses a calcium-dependent lectin domain capable of interacting with glycoconjugates present on microorganisms and leukocytes. Accordingly, we evaluated the extent and localization of SP-D in the lower respiratory tract during Pneumocystis pneumonia in an immunosuppressed rat model and examined its role in modulating interaction of P. carinii with macrophages. We report that SP-D is a major component of the alveolar exudates that typify P. carinii pneumonia and is present bound to the surface of P. carinii organisms in vivo. We further demonstrate that SP- D binds to P. carinii through saccharide-mediated interactions with gpA present on the surface of the organism. Lastly, we show that SP-D augments binding of P. carinii to alveolar macrophages, but does not significantly enhance macrophage phagocytosis of the organism. The interaction of SP-D with gpA represents an additional important component of the host-parasite relationship during P. carinii pneumonia.

Original languageEnglish (US)
Pages (from-to)2699-2710
Number of pages12
JournalJournal of Clinical Investigation
Volume95
Issue number6
StatePublished - Jun 1995

Fingerprint

Pulmonary Surfactant-Associated Protein D
Pneumocystis carinii
Alveolar Macrophages
Pneumocystis Pneumonia
Respiratory System
Macrophages
Host-Parasite Interactions
Glycoconjugates
Exudates and Transudates
Surface Antigens
Mannose
Phagocytosis
Lectins
Glycoproteins
Leukocytes
Calcium

Keywords

  • gpA
  • lectin
  • macrophages
  • Pneumocystis carinii
  • surfactant protein D

ASJC Scopus subject areas

  • Medicine(all)

Cite this

O'Riordan, D. M., Standing, J. E., Kwon, K. Y., Chang, D., Crouch, E. C., & Limper, A. H. (1995). Surfactant protein D interacts with Pneumocystis carinii and mediates organism adherence to alveolar macrophages. Journal of Clinical Investigation, 95(6), 2699-2710.

Surfactant protein D interacts with Pneumocystis carinii and mediates organism adherence to alveolar macrophages. / O'Riordan, Deirdre M.; Standing, Joseph E.; Kwon, Kun Young; Chang, Donald; Crouch, Edmond C.; Limper, Andrew Harold.

In: Journal of Clinical Investigation, Vol. 95, No. 6, 06.1995, p. 2699-2710.

Research output: Contribution to journalArticle

O'Riordan, DM, Standing, JE, Kwon, KY, Chang, D, Crouch, EC & Limper, AH 1995, 'Surfactant protein D interacts with Pneumocystis carinii and mediates organism adherence to alveolar macrophages', Journal of Clinical Investigation, vol. 95, no. 6, pp. 2699-2710.
O'Riordan, Deirdre M. ; Standing, Joseph E. ; Kwon, Kun Young ; Chang, Donald ; Crouch, Edmond C. ; Limper, Andrew Harold. / Surfactant protein D interacts with Pneumocystis carinii and mediates organism adherence to alveolar macrophages. In: Journal of Clinical Investigation. 1995 ; Vol. 95, No. 6. pp. 2699-2710.
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