Suppressor of cytokine signaling 1 counteracts rhesus macaque TRIM5α-induced inhibition of human immunodeficiency virus type-1 production

Sayaka Sukegawa, Ryuta Sakuma, Seiga Ohmine, Hiroaki Takeuchi, Yasuhiro H Ikeda, Shoji Yamaoka

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Old world monkey TRIM5α is a host factor that restricts human immunodeficiency virus type-1 (HIV-1) infection. Previously, we reported that rhesus macaque TRIM5α (RhTRIM5α) restricts HIV-1 production by inducing degradation of precursor Gag. Since suppressor of cytokine signaling 1 (SOCS1) is known to enhance HIV-1 production by rescuing Gag from lysosomal degradation, we examined if SOCS1 is involved in RhTRIM5α-mediated late restriction. Over-expression of SOCS1 restored HIV-1 production in the presence of RhTRIM5α to a level comparable to that in the absence of RhTRIM5α in terms of titer and viral protein expression. Co-immunoprecipitation studies revealed that SOCS1 physically interacted with RhTRIM5α. Over-expression of SOCS1 affected RhTRIM5α expression in a dose-dependent manner, which was not reversed by proteasome inhibitors. In addition, SOCS1 and RhTRIM5α were detected in virus-like particles. These results suggest that SOCS1 alleviates RhTRIM5α-mediated regulation in the late phase of HIV-1 life cycle probably due to the destabilization of RhTRIM5α.

Original languageEnglish (US)
Article numbere109640
JournalPLoS One
Volume9
Issue number10
DOIs
StatePublished - Oct 13 2014

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Human immunodeficiency virus 1
Macaca mulatta
Viruses
HIV-1
cytokines
Cytokines
Degradation
Proteasome Inhibitors
Cercopithecidae
Viral Proteins
virus-like particles
degradation
viral proteins
Life cycle
proteasome endopeptidase complex
Virus Diseases
Life Cycle Stages
Immunoprecipitation
Virion
life cycle (organisms)

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Suppressor of cytokine signaling 1 counteracts rhesus macaque TRIM5α-induced inhibition of human immunodeficiency virus type-1 production. / Sukegawa, Sayaka; Sakuma, Ryuta; Ohmine, Seiga; Takeuchi, Hiroaki; Ikeda, Yasuhiro H; Yamaoka, Shoji.

In: PLoS One, Vol. 9, No. 10, e109640, 13.10.2014.

Research output: Contribution to journalArticle

Sukegawa, Sayaka ; Sakuma, Ryuta ; Ohmine, Seiga ; Takeuchi, Hiroaki ; Ikeda, Yasuhiro H ; Yamaoka, Shoji. / Suppressor of cytokine signaling 1 counteracts rhesus macaque TRIM5α-induced inhibition of human immunodeficiency virus type-1 production. In: PLoS One. 2014 ; Vol. 9, No. 10.
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