Supplementation of ascorbic acid and α-tocopherol prevents arsenic-induced protein oxidation and DNA damage induced by arsenic in rats

Ramanathan D Kadirvel, K. Sundaram, S. Mani, S. Samuel, N. Elango, Chinakkannu Panneerselvam

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Contamination of arsenic in drinking water is associated with several human diseases including cancer. It has been reported that oxidative stress plays a vital role in arsenic-induced biochemical and molecular alterations. The aim of the present study was to improve the understanding of arsenic-induced oxidative damage to proteins and to DNA and the role of antioxidants such as ascorbic acid and α-tocopherol in alleviating arsenic-induced damages in experimental rats. A significant increase in the levels of protein oxidation, DNA strand breaks, and DNA-protein cross-links was observed in blood, liver, and kidney of rats exposed to arsenic (100 ppm in drinking water) for 30 days. Co-administration of ascorbic acid and α-tocopherol to arsenic-exposed rats showed a substantial reduction in the levels of arsenic-induced oxidative products of protein and DNA. The results of this study support that free radical-mediated toxic manifestations of arsenic and also suggest that ascorbic acid and α-tocopherol supplementation can improve the arsenic-induced molecular alterations.

Original languageEnglish (US)
Pages (from-to)939-946
Number of pages8
JournalHuman and Experimental Toxicology
Volume26
Issue number12
DOIs
StatePublished - Dec 2007

Fingerprint

Tocopherols
Arsenic
Ascorbic Acid
DNA Damage
Rats
Oxidation
DNA
Proteins
Drinking Water
DNA Breaks
Oxidative stress
Poisons
Liver
Free Radicals
Oxidative Stress
Contamination
Blood
Antioxidants
Kidney

Keywords

  • Antioxidants
  • Arsenic
  • DNA damage
  • Oxidative stress
  • Protein oxidation

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Supplementation of ascorbic acid and α-tocopherol prevents arsenic-induced protein oxidation and DNA damage induced by arsenic in rats. / Kadirvel, Ramanathan D; Sundaram, K.; Mani, S.; Samuel, S.; Elango, N.; Panneerselvam, Chinakkannu.

In: Human and Experimental Toxicology, Vol. 26, No. 12, 12.2007, p. 939-946.

Research output: Contribution to journalArticle

Kadirvel, Ramanathan D ; Sundaram, K. ; Mani, S. ; Samuel, S. ; Elango, N. ; Panneerselvam, Chinakkannu. / Supplementation of ascorbic acid and α-tocopherol prevents arsenic-induced protein oxidation and DNA damage induced by arsenic in rats. In: Human and Experimental Toxicology. 2007 ; Vol. 26, No. 12. pp. 939-946.
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