Superoxide anion is an endothelium-derived contracting factor

Zvonimir S Katusic, P. M. Vanhoutte

Research output: Contribution to journalArticle

362 Citations (Scopus)

Abstract

The calcium ionophore A23187 causes endothelium-dependent contractions in canine basilar arteries. Removal of the endothelium, or treatment with indomethacin or superoxide dismutase (SOD), prevented the endothelium-dependent excitatory effect of the calcium ionophore. Catalase and deferoxamine were without effect. Superoxide anion generated by xanthine plus xanthine oxidase in the presence of catalase caused contractions of the vascular smooth muscle, which were abolished by SOD or heat inactivation of xanthine oxidase. The A23187-induced production of prostaglandins F(2α) and E2 and thromboxane B2 was abolished by the removal of endothelium and by treatment with indomethacin but was not affected by the presence of SOD plus catalase. These observations are consistent with the hypothesis that superoxide anion, rather than prostaglandins generated by hydroperoxidase activity of cyclooxygenase, is an endothelium-derived contracting factor in canine cerebral arteries.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume257
Issue number1
StatePublished - 1989

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Superoxides
Endothelium
Catalase
Superoxide Dismutase
Calcium Ionophores
Xanthine Oxidase
Calcimycin
Indomethacin
Canidae
Thromboxane B2
Basilar Artery
Xanthine
Deferoxamine
Cerebral Arteries
Prostaglandins F
Prostaglandin-Endoperoxide Synthases
Vascular Smooth Muscle
Dinoprostone
Hot Temperature

ASJC Scopus subject areas

  • Physiology

Cite this

Superoxide anion is an endothelium-derived contracting factor. / Katusic, Zvonimir S; Vanhoutte, P. M.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 257, No. 1, 1989.

Research output: Contribution to journalArticle

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