Supercritical Carbon Dioxide–Based Sterilization of Decellularized Heart Valves

Ryan S. Hennessy; Soumen Jana; Brandon J. Tefft; Meghana R. Helder; Melissa D. Young; Rebecca R. Hennessy; Nicholas J. Stoyles; Amir Lerman

doi:10.1016/j.jacbts.2016.08.009

Supercritical Carbon Dioxide–Based Sterilization of Decellularized Heart Valves

Ryan S. Hennessy, Soumen Jana, Brandon J. Tefft, Meghana R. Helder, Melissa D. Young, Rebecca R. Hennessy, Nicholas J. Stoyles, Amir Lerman

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

Sterilization of grafts is essential. Supercritical carbon dioxide, electrolyzed water, gamma radiation, ethanol-peracetic acid, and hydrogen peroxide techniques were compared for impact on sterility and mechanical integrity of porcine decellularized aortic valves. Ethanol-peracetic acid– and supercritical carbon dioxide–treated valves were found to be sterile using histology, microbe culture, and electron microscopy assays. The cusp tensile properties of supercritical carbon dioxide–treated valves were higher compared with valves treated with other techniques. Superior sterility and integrity was found in the decellularized valves treated with supercritical carbon dioxide sterilization. This sterilization technique may hold promise for other decellularized soft tissues.

Original language	English (US)
Pages (from-to)	71-84
Number of pages	14
Journal	JACC: Basic to Translational Science
Volume	2
Issue number	1
DOIs	https://doi.org/10.1016/j.jacbts.2016.08.009
State	Published - 2017

Keywords

decellularized
decontamination
heart valve
tensile properties
tissue engineering

ASJC Scopus subject areas

Cardiology and Cardiovascular Medicine

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10.1016/j.jacbts.2016.08.009

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Supercritical Carbon Dioxide–Based Sterilization of Decellularized Heart Valves. / Hennessy, Ryan S.; Jana, Soumen; Tefft, Brandon J.; Helder, Meghana R.; Young, Melissa D.; Hennessy, Rebecca R.; Stoyles, Nicholas J.; Lerman, Amir.

In: JACC: Basic to Translational Science, Vol. 2, No. 1, 2017, p. 71-84.

Research output: Contribution to journal › Article › peer-review

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abstract = "Sterilization of grafts is essential. Supercritical carbon dioxide, electrolyzed water, gamma radiation, ethanol-peracetic acid, and hydrogen peroxide techniques were compared for impact on sterility and mechanical integrity of porcine decellularized aortic valves. Ethanol-peracetic acid– and supercritical carbon dioxide–treated valves were found to be sterile using histology, microbe culture, and electron microscopy assays. The cusp tensile properties of supercritical carbon dioxide–treated valves were higher compared with valves treated with other techniques. Superior sterility and integrity was found in the decellularized valves treated with supercritical carbon dioxide sterilization. This sterilization technique may hold promise for other decellularized soft tissues.",

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AU - Hennessy, Rebecca R.

AU - Stoyles, Nicholas J.

AU - Lerman, Amir

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AB - Sterilization of grafts is essential. Supercritical carbon dioxide, electrolyzed water, gamma radiation, ethanol-peracetic acid, and hydrogen peroxide techniques were compared for impact on sterility and mechanical integrity of porcine decellularized aortic valves. Ethanol-peracetic acid– and supercritical carbon dioxide–treated valves were found to be sterile using histology, microbe culture, and electron microscopy assays. The cusp tensile properties of supercritical carbon dioxide–treated valves were higher compared with valves treated with other techniques. Superior sterility and integrity was found in the decellularized valves treated with supercritical carbon dioxide sterilization. This sterilization technique may hold promise for other decellularized soft tissues.

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Supercritical Carbon Dioxide–Based Sterilization of Decellularized Heart Valves

Abstract

Keywords

ASJC Scopus subject areas

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