Supercritical Carbon Dioxide–Based Sterilization of Decellularized Heart Valves

Ryan S. Hennessy, Soumen Jana, Brandon J. Tefft, Meghana R. Helder, Melissa D Young, Rebecca R. Hennessy, Nicholas J. Stoyles, Amir Lerman

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Sterilization of grafts is essential. Supercritical carbon dioxide, electrolyzed water, gamma radiation, ethanol-peracetic acid, and hydrogen peroxide techniques were compared for impact on sterility and mechanical integrity of porcine decellularized aortic valves. Ethanol-peracetic acid– and supercritical carbon dioxide–treated valves were found to be sterile using histology, microbe culture, and electron microscopy assays. The cusp tensile properties of supercritical carbon dioxide–treated valves were higher compared with valves treated with other techniques. Superior sterility and integrity was found in the decellularized valves treated with supercritical carbon dioxide sterilization. This sterilization technique may hold promise for other decellularized soft tissues.

Original languageEnglish (US)
Pages (from-to)71-84
Number of pages14
JournalJACC: Basic to Translational Science
Volume2
Issue number1
DOIs
StatePublished - 2017

Fingerprint

Heart Valves
Peracetic Acid
Carbon
Carbon Dioxide
Infertility
Ethanol
Gamma Rays
Aortic Valve
Hydrogen Peroxide
Histology
Electron Microscopy
Swine
Transplants
Water

Keywords

  • decellularized
  • decontamination
  • heart valve
  • tensile properties
  • tissue engineering

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Supercritical Carbon Dioxide–Based Sterilization of Decellularized Heart Valves. / Hennessy, Ryan S.; Jana, Soumen; Tefft, Brandon J.; Helder, Meghana R.; Young, Melissa D; Hennessy, Rebecca R.; Stoyles, Nicholas J.; Lerman, Amir.

In: JACC: Basic to Translational Science, Vol. 2, No. 1, 2017, p. 71-84.

Research output: Contribution to journalArticle

Hennessy, Ryan S. ; Jana, Soumen ; Tefft, Brandon J. ; Helder, Meghana R. ; Young, Melissa D ; Hennessy, Rebecca R. ; Stoyles, Nicholas J. ; Lerman, Amir. / Supercritical Carbon Dioxide–Based Sterilization of Decellularized Heart Valves. In: JACC: Basic to Translational Science. 2017 ; Vol. 2, No. 1. pp. 71-84.
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