Abstract
Sterilization of grafts is essential. Supercritical carbon dioxide, electrolyzed water, gamma radiation, ethanol-peracetic acid, and hydrogen peroxide techniques were compared for impact on sterility and mechanical integrity of porcine decellularized aortic valves. Ethanol-peracetic acid– and supercritical carbon dioxide–treated valves were found to be sterile using histology, microbe culture, and electron microscopy assays. The cusp tensile properties of supercritical carbon dioxide–treated valves were higher compared with valves treated with other techniques. Superior sterility and integrity was found in the decellularized valves treated with supercritical carbon dioxide sterilization. This sterilization technique may hold promise for other decellularized soft tissues.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 71-84 |
| Number of pages | 14 |
| Journal | JACC: Basic to Translational Science |
| Volume | 2 |
| Issue number | 1 |
| DOIs | |
| State | Published - 2017 |
Fingerprint
Keywords
- decellularized
- decontamination
- heart valve
- tensile properties
- tissue engineering
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine
Cite this
Supercritical Carbon Dioxide–Based Sterilization of Decellularized Heart Valves. / Hennessy, Ryan S.; Jana, Soumen; Tefft, Brandon J.; Helder, Meghana R.; Young, Melissa D; Hennessy, Rebecca R.; Stoyles, Nicholas J.; Lerman, Amir.
In: JACC: Basic to Translational Science, Vol. 2, No. 1, 2017, p. 71-84.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Supercritical Carbon Dioxide–Based Sterilization of Decellularized Heart Valves
AU - Hennessy, Ryan S.
AU - Jana, Soumen
AU - Tefft, Brandon J.
AU - Helder, Meghana R.
AU - Young, Melissa D
AU - Hennessy, Rebecca R.
AU - Stoyles, Nicholas J.
AU - Lerman, Amir
PY - 2017
Y1 - 2017
N2 - Sterilization of grafts is essential. Supercritical carbon dioxide, electrolyzed water, gamma radiation, ethanol-peracetic acid, and hydrogen peroxide techniques were compared for impact on sterility and mechanical integrity of porcine decellularized aortic valves. Ethanol-peracetic acid– and supercritical carbon dioxide–treated valves were found to be sterile using histology, microbe culture, and electron microscopy assays. The cusp tensile properties of supercritical carbon dioxide–treated valves were higher compared with valves treated with other techniques. Superior sterility and integrity was found in the decellularized valves treated with supercritical carbon dioxide sterilization. This sterilization technique may hold promise for other decellularized soft tissues.
AB - Sterilization of grafts is essential. Supercritical carbon dioxide, electrolyzed water, gamma radiation, ethanol-peracetic acid, and hydrogen peroxide techniques were compared for impact on sterility and mechanical integrity of porcine decellularized aortic valves. Ethanol-peracetic acid– and supercritical carbon dioxide–treated valves were found to be sterile using histology, microbe culture, and electron microscopy assays. The cusp tensile properties of supercritical carbon dioxide–treated valves were higher compared with valves treated with other techniques. Superior sterility and integrity was found in the decellularized valves treated with supercritical carbon dioxide sterilization. This sterilization technique may hold promise for other decellularized soft tissues.
KW - decellularized
KW - decontamination
KW - heart valve
KW - tensile properties
KW - tissue engineering
UR - http://www.scopus.com/inward/record.url?scp=85020655291&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85020655291&partnerID=8YFLogxK
U2 - 10.1016/j.jacbts.2016.08.009
DO - 10.1016/j.jacbts.2016.08.009
M3 - Article
AN - SCOPUS:85020655291
VL - 2
SP - 71
EP - 84
JO - JACC: Basic to Translational Science
JF - JACC: Basic to Translational Science
SN - 2452-302X
IS - 1
ER -