TY - JOUR
T1 - Superantigen profiling of Staphylococcus aureus infective endocarditis isolates
AU - Chung, Jin Won
AU - Karau, Melissa J.
AU - Greenwood-Quaintance, Kerryl E.
AU - Ballard, Alessandro D.
AU - Tilahun, Ashenafi
AU - Khaleghi, Shahryar Rostamkolaei
AU - David, Chella S.
AU - Patel, Robin
AU - Rajagopalan, Govindarajan
N1 - Funding Information:
This study was funded by NIH grants AI101172 to GR and AI68741 to GR and CSD.
PY - 2014/6
Y1 - 2014/6
N2 - The frequency of superantigen production among Staphylococcus aureus isolates associated with endocarditis is not well defined. We tested 154 S. aureus isolates from definite infective endocarditis cases for the presence of staphylococcal enterotoxins A-E, H, and TSST-1 by PCR, enzyme-linked immunosorbent assay, and using an HLA-DR3 transgenic mouse splenocyte proliferation assay. Sixty-three isolates (50.8%) tested positive for at least 1 superantigen gene, with 21 (16.9%) testing positive for more than 2. tst (28.6%) was most common, followed by seb (27%), sea (22.2%), sed (20.6%), see (17.5%), and sec (11.1%). Of 41 methicillin-resistant S. aureus, 21 had superantigen genes, with sed being more frequently detected in this group compared to methicillin-susceptible S. aureus (P < 0.05). Superantigen genes were not associated with mortality (P = 0.81). 75% of PCR-positive isolates induced robust splenocyte proliferation. Overall, more than half of S. aureus isolates causing endocarditis carry superantigen genes, of which most are functional.
AB - The frequency of superantigen production among Staphylococcus aureus isolates associated with endocarditis is not well defined. We tested 154 S. aureus isolates from definite infective endocarditis cases for the presence of staphylococcal enterotoxins A-E, H, and TSST-1 by PCR, enzyme-linked immunosorbent assay, and using an HLA-DR3 transgenic mouse splenocyte proliferation assay. Sixty-three isolates (50.8%) tested positive for at least 1 superantigen gene, with 21 (16.9%) testing positive for more than 2. tst (28.6%) was most common, followed by seb (27%), sea (22.2%), sed (20.6%), see (17.5%), and sec (11.1%). Of 41 methicillin-resistant S. aureus, 21 had superantigen genes, with sed being more frequently detected in this group compared to methicillin-susceptible S. aureus (P < 0.05). Superantigen genes were not associated with mortality (P = 0.81). 75% of PCR-positive isolates induced robust splenocyte proliferation. Overall, more than half of S. aureus isolates causing endocarditis carry superantigen genes, of which most are functional.
KW - Infective endocarditis
KW - S. aureus
KW - Superantigen
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U2 - 10.1016/j.diagmicrobio.2014.03.009
DO - 10.1016/j.diagmicrobio.2014.03.009
M3 - Article
C2 - 24745820
AN - SCOPUS:84900520113
VL - 79
SP - 119
EP - 124
JO - Diagnostic Microbiology and Infectious Disease
JF - Diagnostic Microbiology and Infectious Disease
SN - 0732-8893
IS - 2
ER -