Superantigen profiling of Staphylococcus aureus infective endocarditis isolates

Jin Won Chung, Melissa J. Karau, Kerryl E. Greenwood-Quaintance, Alessandro D. Ballard, Ashenafi Tilahun, Shahryar Rostamkolaei Khaleghi, Chella S. David, Robin Patel, Govindarajan Rajagopalan

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The frequency of superantigen production among Staphylococcus aureus isolates associated with endocarditis is not well defined. We tested 154 S. aureus isolates from definite infective endocarditis cases for the presence of staphylococcal enterotoxins A-E, H, and TSST-1 by PCR, enzyme-linked immunosorbent assay, and using an HLA-DR3 transgenic mouse splenocyte proliferation assay. Sixty-three isolates (50.8%) tested positive for at least 1 superantigen gene, with 21 (16.9%) testing positive for more than 2. tst (28.6%) was most common, followed by seb (27%), sea (22.2%), sed (20.6%), see (17.5%), and sec (11.1%). Of 41 methicillin-resistant S. aureus, 21 had superantigen genes, with sed being more frequently detected in this group compared to methicillin-susceptible S. aureus (P < 0.05). Superantigen genes were not associated with mortality (P = 0.81). 75% of PCR-positive isolates induced robust splenocyte proliferation. Overall, more than half of S. aureus isolates causing endocarditis carry superantigen genes, of which most are functional.

Original languageEnglish (US)
Pages (from-to)119-124
Number of pages6
JournalDiagnostic Microbiology and Infectious Disease
Volume79
Issue number2
DOIs
StatePublished - 2014

Fingerprint

Superantigens
Endocarditis
Staphylococcus aureus
Genes
HLA-DR3 Antigen
Polymerase Chain Reaction
Methicillin
Methicillin-Resistant Staphylococcus aureus
Oceans and Seas
Transgenic Mice
Enzyme-Linked Immunosorbent Assay
Mortality

Keywords

  • Infective endocarditis
  • S. aureus
  • Superantigen

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases
  • Medicine(all)

Cite this

Chung, J. W., Karau, M. J., Greenwood-Quaintance, K. E., Ballard, A. D., Tilahun, A., Khaleghi, S. R., ... Rajagopalan, G. (2014). Superantigen profiling of Staphylococcus aureus infective endocarditis isolates. Diagnostic Microbiology and Infectious Disease, 79(2), 119-124. https://doi.org/10.1016/j.diagmicrobio.2014.03.009

Superantigen profiling of Staphylococcus aureus infective endocarditis isolates. / Chung, Jin Won; Karau, Melissa J.; Greenwood-Quaintance, Kerryl E.; Ballard, Alessandro D.; Tilahun, Ashenafi; Khaleghi, Shahryar Rostamkolaei; David, Chella S.; Patel, Robin; Rajagopalan, Govindarajan.

In: Diagnostic Microbiology and Infectious Disease, Vol. 79, No. 2, 2014, p. 119-124.

Research output: Contribution to journalArticle

Chung, JW, Karau, MJ, Greenwood-Quaintance, KE, Ballard, AD, Tilahun, A, Khaleghi, SR, David, CS, Patel, R & Rajagopalan, G 2014, 'Superantigen profiling of Staphylococcus aureus infective endocarditis isolates', Diagnostic Microbiology and Infectious Disease, vol. 79, no. 2, pp. 119-124. https://doi.org/10.1016/j.diagmicrobio.2014.03.009
Chung, Jin Won ; Karau, Melissa J. ; Greenwood-Quaintance, Kerryl E. ; Ballard, Alessandro D. ; Tilahun, Ashenafi ; Khaleghi, Shahryar Rostamkolaei ; David, Chella S. ; Patel, Robin ; Rajagopalan, Govindarajan. / Superantigen profiling of Staphylococcus aureus infective endocarditis isolates. In: Diagnostic Microbiology and Infectious Disease. 2014 ; Vol. 79, No. 2. pp. 119-124.
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