Superantigen-producing Staphylococcus aureus elicits systemic immune activation in a murine wound colonization model

Choon K. Kim, Melissa J. Karau, Kerryl E. Greenwood-Quaintance, Ashenafi Y. Tilahun, Ashton Krogman, Chella S. David, Bobbi S. Pritt, Robin Patel, Govindarajan Rajagopalan

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Staphylococcus aureus, the most common cause of wound infection, produces several exotoxins, including superantigens (SAgs). SAgs are the potent activators of the immune system. Given this unique property, we hypothesized that SAgs produced by S. aureus in wounds would have local, as well as systemic immunologic effects. We tested our hypothesis using a novel staphylococcal skin wound infection model in transgenic mice expressing HLA-DR3. Skin wounds were left uninfected or colonized with S. aureus strains producing SAgs or an isogenic strain not producing any SAg. Animals with wounds challenged with SAg-producing S. aureus had increased morbidity and lower serum IL-17 levels compared to those challenged with the SAg non-producing S. aureus (p = 0.027 and p = 0.032, respectively). At Day 8 following microbial challenge, compared to mice with uninfected wounds, the proportion of Vβ8+CD4+ T cells was increased, while the proportion of Vβ8+CD8+ T cells was decreased only in the spleens of mice challenged with SAg-producing S. aureus (p <0.001). No such changes were measured in mice challenged with SAg non-producing S. aureus. Lungs, livers and kidneys from mice challenged with SAg-producing, but not SAg non-producing, S. aureus showed inflammatory changes. Overall, SAg-mediated systemic immune activation in wounds harboring S. aureus may have clinical implications.

Original languageEnglish (US)
Pages (from-to)5308-5319
Number of pages12
JournalToxins
Volume7
Issue number12
DOIs
StatePublished - Dec 8 2015

Fingerprint

Superantigens
Staphylococcus aureus
Chemical activation
T-cells
Wounds and Injuries
Skin
HLA-DR3 Antigen
Exotoxins
Interleukin-17
Immune system
Liver
Wound Infection
Animals
Staphylococcal Skin Infections
T-Lymphocytes
Transgenic Mice
Immune System
Spleen
Morbidity
Kidney

Keywords

  • HLA class II transgenic mice
  • Inflammation
  • Skin infection
  • Staphylococcus aureus
  • Superantigen
  • Wound healing

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Kim, C. K., Karau, M. J., Greenwood-Quaintance, K. E., Tilahun, A. Y., Krogman, A., David, C. S., ... Rajagopalan, G. (2015). Superantigen-producing Staphylococcus aureus elicits systemic immune activation in a murine wound colonization model. Toxins, 7(12), 5308-5319. https://doi.org/10.3390/toxins7124886

Superantigen-producing Staphylococcus aureus elicits systemic immune activation in a murine wound colonization model. / Kim, Choon K.; Karau, Melissa J.; Greenwood-Quaintance, Kerryl E.; Tilahun, Ashenafi Y.; Krogman, Ashton; David, Chella S.; Pritt, Bobbi S.; Patel, Robin; Rajagopalan, Govindarajan.

In: Toxins, Vol. 7, No. 12, 08.12.2015, p. 5308-5319.

Research output: Contribution to journalArticle

Kim, CK, Karau, MJ, Greenwood-Quaintance, KE, Tilahun, AY, Krogman, A, David, CS, Pritt, BS, Patel, R & Rajagopalan, G 2015, 'Superantigen-producing Staphylococcus aureus elicits systemic immune activation in a murine wound colonization model', Toxins, vol. 7, no. 12, pp. 5308-5319. https://doi.org/10.3390/toxins7124886
Kim CK, Karau MJ, Greenwood-Quaintance KE, Tilahun AY, Krogman A, David CS et al. Superantigen-producing Staphylococcus aureus elicits systemic immune activation in a murine wound colonization model. Toxins. 2015 Dec 8;7(12):5308-5319. https://doi.org/10.3390/toxins7124886
Kim, Choon K. ; Karau, Melissa J. ; Greenwood-Quaintance, Kerryl E. ; Tilahun, Ashenafi Y. ; Krogman, Ashton ; David, Chella S. ; Pritt, Bobbi S. ; Patel, Robin ; Rajagopalan, Govindarajan. / Superantigen-producing Staphylococcus aureus elicits systemic immune activation in a murine wound colonization model. In: Toxins. 2015 ; Vol. 7, No. 12. pp. 5308-5319.
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