31P NMR assessment of orthotopic liver rejection in a rat model

John L. Bowers; Katsunori Kawano; Kenneth R. Metz; Kenichi Teramoto; Ann McCullough; Melvin E. Clouse

doi:10.1002/mrm.1910320203

³¹P NMR assessment of orthotopic liver rejection in a rat model

John L. Bowers, Katsunori Kawano, Kenneth R. Metz, Kenichi Teramoto, Ann McCullough, Melvin E. Clouse

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

³¹P NMR spectroscopy was used serially to study rejecting (ACI→LEW) and nonrejecting (ACl→ACl) orthotopic liver transplants in rats. Recipients were evaluated on post‐transplant days 1,3, 5, 7, 9, and 11. The relative changes in phosphomonoester (PME), inorganic phosphate (P_i), high‐energy phosphates and pH were studied. The earliest significant difference between the rejecting and nonrejecting groups was a decrease in the α‐NTP peak area on Day 5. This was followed by significant decreases in β‐NTP and pH, and increases in PME and P_i on Day 7. High‐resolution ³¹P NMR spectra of perchloric acid extracts demonstrated the PME increase to be due mainly to elevated phosphoethanolamine. Using the parameter (P_i + PME)/(α + β + γ‐NTP), rejecting livers were distinguished from nonrejecting livers at a moderate stage of rejection.

Original language	English (US)
Pages (from-to)	164-169
Number of pages	6
Journal	Magnetic Resonance in Medicine
Volume	32
Issue number	2
DOIs	https://doi.org/10.1002/mrm.1910320203
State	Published - Aug 1994

Keywords

P NMR
liver
rejection
transplantation

ASJC Scopus subject areas

Radiology Nuclear Medicine and imaging

Access to Document

10.1002/mrm.1910320203

Cite this

@article{933dabddd90f41788d366f629eedb18e,

title = "31P NMR assessment of orthotopic liver rejection in a rat model",

abstract = "31P NMR spectroscopy was used serially to study rejecting (ACI→LEW) and nonrejecting (ACl→ACl) orthotopic liver transplants in rats. Recipients were evaluated on post‐transplant days 1,3, 5, 7, 9, and 11. The relative changes in phosphomonoester (PME), inorganic phosphate (Pi), high‐energy phosphates and pH were studied. The earliest significant difference between the rejecting and nonrejecting groups was a decrease in the α‐NTP peak area on Day 5. This was followed by significant decreases in β‐NTP and pH, and increases in PME and Pi on Day 7. High‐resolution 31P NMR spectra of perchloric acid extracts demonstrated the PME increase to be due mainly to elevated phosphoethanolamine. Using the parameter (Pi + PME)/(α + β + γ‐NTP), rejecting livers were distinguished from nonrejecting livers at a moderate stage of rejection.",

keywords = "P NMR, liver, rejection, transplantation",

author = "Bowers, {John L.} and Katsunori Kawano and Metz, {Kenneth R.} and Kenichi Teramoto and Ann McCullough and Clouse, {Melvin E.}",

year = "1994",

month = aug,

doi = "10.1002/mrm.1910320203",

language = "English (US)",

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T1 - 31P NMR assessment of orthotopic liver rejection in a rat model

AU - Bowers, John L.

AU - Kawano, Katsunori

AU - Metz, Kenneth R.

AU - Teramoto, Kenichi

AU - McCullough, Ann

AU - Clouse, Melvin E.

PY - 1994/8

Y1 - 1994/8

N2 - 31P NMR spectroscopy was used serially to study rejecting (ACI→LEW) and nonrejecting (ACl→ACl) orthotopic liver transplants in rats. Recipients were evaluated on post‐transplant days 1,3, 5, 7, 9, and 11. The relative changes in phosphomonoester (PME), inorganic phosphate (Pi), high‐energy phosphates and pH were studied. The earliest significant difference between the rejecting and nonrejecting groups was a decrease in the α‐NTP peak area on Day 5. This was followed by significant decreases in β‐NTP and pH, and increases in PME and Pi on Day 7. High‐resolution 31P NMR spectra of perchloric acid extracts demonstrated the PME increase to be due mainly to elevated phosphoethanolamine. Using the parameter (Pi + PME)/(α + β + γ‐NTP), rejecting livers were distinguished from nonrejecting livers at a moderate stage of rejection.

AB - 31P NMR spectroscopy was used serially to study rejecting (ACI→LEW) and nonrejecting (ACl→ACl) orthotopic liver transplants in rats. Recipients were evaluated on post‐transplant days 1,3, 5, 7, 9, and 11. The relative changes in phosphomonoester (PME), inorganic phosphate (Pi), high‐energy phosphates and pH were studied. The earliest significant difference between the rejecting and nonrejecting groups was a decrease in the α‐NTP peak area on Day 5. This was followed by significant decreases in β‐NTP and pH, and increases in PME and Pi on Day 7. High‐resolution 31P NMR spectra of perchloric acid extracts demonstrated the PME increase to be due mainly to elevated phosphoethanolamine. Using the parameter (Pi + PME)/(α + β + γ‐NTP), rejecting livers were distinguished from nonrejecting livers at a moderate stage of rejection.

KW - P NMR

KW - liver

KW - rejection

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