31P NMR assessment of orthotopic liver rejection in a rat model

John L. Bowers, Katsunori Kawano, Kenneth R. Metz, Kenichi Teramoto, Ann McCullough, Melvin E. Clouse

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

31P NMR spectroscopy was used serially to study rejecting (ACI→LEW) and nonrejecting (ACl→ACl) orthotopic liver transplants in rats. Recipients were evaluated on post‐transplant days 1,3, 5, 7, 9, and 11. The relative changes in phosphomonoester (PME), inorganic phosphate (Pi), high‐energy phosphates and pH were studied. The earliest significant difference between the rejecting and nonrejecting groups was a decrease in the α‐NTP peak area on Day 5. This was followed by significant decreases in β‐NTP and pH, and increases in PME and Pi on Day 7. High‐resolution 31P NMR spectra of perchloric acid extracts demonstrated the PME increase to be due mainly to elevated phosphoethanolamine. Using the parameter (Pi + PME)/(α + β + γ‐NTP), rejecting livers were distinguished from nonrejecting livers at a moderate stage of rejection.

Original languageEnglish (US)
Pages (from-to)164-169
Number of pages6
JournalMagnetic Resonance in Medicine
Volume32
Issue number2
DOIs
StatePublished - Aug 1994

Keywords

  • P NMR
  • liver
  • rejection
  • transplantation

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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