18F-FDG labelling of human leukocytes

L. A. Forstrom, B. P. Mullan, J. C. Hung, Val Lowe, L. M. Thorson

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Abstract

Radiolabelled leukocytes are useful for the imaging of inflammation and infection, and 18F-fluorodeoxyglucose (18F-FDG) is known to concentrate in metabolically active cells. We evaluated the feasibility of leukocyte labelling with 18F-FDG using ACD and heparin anticoagulants at 20°C and 37°C, with and without gentle mixing during incubation. With leukocytes (WBC) harvested from 20 ml blood, studies were performed using 18F-FDG in concentrations of 3.7-74 MBq (0.1-2.0 mCi). 18F-FDG WBC stability in platelet-poor plasma was assessed at 1-4 h. Satisfactory labelling efficiency was achieved with incubation in heparin-saline at 37°C for 30 min (62.7±1.6%), and was further enhanced by mixing during incubation (78.1±3.9%). Cell labelling was predominantly of granulocytes (78.5±1.4%). 18F-FDG WBC was relatively stable in platelet-poor plasma for up to 4 h, and no cell staining was observed in viability studies using trypan blue. These results indicate the feasibility of leukocyte labelling with 18F-FDG, providing an approach that may be useful in PET imaging of inflammation and infection. (

Original languageEnglish (US)
Pages (from-to)691-694
Number of pages4
JournalNuclear Medicine Communications
Volume21
Issue number7
StatePublished - 2000

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ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

Forstrom, L. A., Mullan, B. P., Hung, J. C., Lowe, V., & Thorson, L. M. (2000). 18F-FDG labelling of human leukocytes. Nuclear Medicine Communications, 21(7), 691-694.