Subunit stoichiometry of the epithelial sodium channel

Farhad Kosari, Shaohu Sheng, Jinqing Li, Don On D Mak, J. Kevin Foskett, Thomas R. Kleyman

Research output: Contribution to journalArticle

187 Citations (Scopus)

Abstract

The epithelial Na+ ChAnnel (ENaC) mediates Na+ reabsorption in a variety of epithelial tissues. ENaC is composed of three homologous subunits, termed α, β, and γ. All three subunits participate in channel formation as the absence of any one subunit results in a significant reduction or complete abrogation of Na+ current expression in Xenopus oocytes. To determine the subunit stoichiometry, a biophysical assay was employed utilizing mutant subunits that display significant differences in sensitivity to channel blockers from the wild type channel. Our results indicate that ENaC is a tetrameric channel with an α2βγ stoichiometry, similar to that reported for other cation selective channels, such as K(r), K(ir), as well as voltage- gated Na+ and Ca2+ channels that have 4-fold internal symmetry.

Original languageEnglish (US)
Pages (from-to)13469-13474
Number of pages6
JournalJournal of Biological Chemistry
Volume273
Issue number22
DOIs
StatePublished - May 29 1998
Externally publishedYes

Fingerprint

Epithelial Sodium Channels
Stoichiometry
Xenopus
Oocytes
Cations
Assays
Epithelium
Tissue
Electric potential

ASJC Scopus subject areas

  • Biochemistry

Cite this

Kosari, F., Sheng, S., Li, J., Mak, D. O. D., Foskett, J. K., & Kleyman, T. R. (1998). Subunit stoichiometry of the epithelial sodium channel. Journal of Biological Chemistry, 273(22), 13469-13474. https://doi.org/10.1074/jbc.273.22.13469

Subunit stoichiometry of the epithelial sodium channel. / Kosari, Farhad; Sheng, Shaohu; Li, Jinqing; Mak, Don On D; Foskett, J. Kevin; Kleyman, Thomas R.

In: Journal of Biological Chemistry, Vol. 273, No. 22, 29.05.1998, p. 13469-13474.

Research output: Contribution to journalArticle

Kosari, F, Sheng, S, Li, J, Mak, DOD, Foskett, JK & Kleyman, TR 1998, 'Subunit stoichiometry of the epithelial sodium channel', Journal of Biological Chemistry, vol. 273, no. 22, pp. 13469-13474. https://doi.org/10.1074/jbc.273.22.13469
Kosari, Farhad ; Sheng, Shaohu ; Li, Jinqing ; Mak, Don On D ; Foskett, J. Kevin ; Kleyman, Thomas R. / Subunit stoichiometry of the epithelial sodium channel. In: Journal of Biological Chemistry. 1998 ; Vol. 273, No. 22. pp. 13469-13474.
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