TY - JOUR
T1 - Subunit stoichiometry of the epithelial sodium channel
AU - Kosari, Farhad
AU - Sheng, Shaohu
AU - Li, Jinqing
AU - Mak, Don On D.
AU - Foskett, J. Kevin
AU - Kleyman, Thomas R.
PY - 1998/5/29
Y1 - 1998/5/29
N2 - The epithelial Na+ ChAnnel (ENaC) mediates Na+ reabsorption in a variety of epithelial tissues. ENaC is composed of three homologous subunits, termed α, β, and γ. All three subunits participate in channel formation as the absence of any one subunit results in a significant reduction or complete abrogation of Na+ current expression in Xenopus oocytes. To determine the subunit stoichiometry, a biophysical assay was employed utilizing mutant subunits that display significant differences in sensitivity to channel blockers from the wild type channel. Our results indicate that ENaC is a tetrameric channel with an α2βγ stoichiometry, similar to that reported for other cation selective channels, such as K(r), K(ir), as well as voltage- gated Na+ and Ca2+ channels that have 4-fold internal symmetry.
AB - The epithelial Na+ ChAnnel (ENaC) mediates Na+ reabsorption in a variety of epithelial tissues. ENaC is composed of three homologous subunits, termed α, β, and γ. All three subunits participate in channel formation as the absence of any one subunit results in a significant reduction or complete abrogation of Na+ current expression in Xenopus oocytes. To determine the subunit stoichiometry, a biophysical assay was employed utilizing mutant subunits that display significant differences in sensitivity to channel blockers from the wild type channel. Our results indicate that ENaC is a tetrameric channel with an α2βγ stoichiometry, similar to that reported for other cation selective channels, such as K(r), K(ir), as well as voltage- gated Na+ and Ca2+ channels that have 4-fold internal symmetry.
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U2 - 10.1074/jbc.273.22.13469
DO - 10.1074/jbc.273.22.13469
M3 - Article
C2 - 9593680
AN - SCOPUS:0032577465
SN - 0021-9258
VL - 273
SP - 13469
EP - 13474
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 22
ER -