Subunit composition of AMPD varies in response to changes in AMPD1 and AMPD3 gene expression in skeletal muscle.

F David Fortuin, T. Morisaki, E. W. Holmes

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Adenosine monophosphate deaminase (AMPD), a central enzyme in energy metabolism in skeletal muscle, is encoded by a multigene family in higher eukaryotes. Denervation was used as a stimulus to induce a change in fiber type composition of rat gastrocnemius muscle and, consequently, gene expression. Specific antisera and nucleic acid probes were used to assess changes in expression of the AMPD1 and AMPD3 genes. Total AMPD activity in denervated skeletal muscle increased by 34%. The composition of the AMPD tetrameric holoenzyme was altered in two ways: The percentage of AMPD holoenzyme molecules consisting of one or more AMPD3 subunits increased three-fold, and the percentage of AMPD1 mRNA that excludes exon 2-encoded sequences doubled. These results suggest that expression of the AMPD1 and AMPD3 genes may be coordinated in myocytes to effect production of an AMPD holoenzyme of varying subunit composition.

Original languageEnglish (US)
Pages (from-to)329-333
Number of pages5
JournalProceedings of the Association of American Physicians
Volume108
Issue number4
StatePublished - Jan 1 1996
Externally publishedYes

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Adenosine Deaminase
Adenosine Monophosphate
Skeletal Muscle
Holoenzymes
Gene Expression
Nucleic Acid Probes
Denervation
Multigene Family
Eukaryota
Muscle Cells
Energy Metabolism
Genes
Immune Sera
Exons
Messenger RNA
Enzymes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Subunit composition of AMPD varies in response to changes in AMPD1 and AMPD3 gene expression in skeletal muscle. / Fortuin, F David; Morisaki, T.; Holmes, E. W.

In: Proceedings of the Association of American Physicians, Vol. 108, No. 4, 01.01.1996, p. 329-333.

Research output: Contribution to journalArticle

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