Subsynovial connective tissue is sensitive to surgical interventions in a rabbit model of carpal tunnel syndrome

Yu Long Sun, Tamami Moriya, Chunfeng D Zhao, Ramona L. Kirk, Takako Chikenji, Sandra M. Passe, Kai Nan An, Peter C Amadio

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The most common histological finding in carpal tunnel syndrome (CTS) is non-inflammatory fibrosis and thickening of the subsynovial connective tissue (SSCT) in the tunnel. While the cause of SSCT fibrosis and the relationship of SSCT fibrosis and CTS are unknown, one hypothesis is that SSCT injury causes fibrosis, and that the fibrosis then leads to CTS. We investigated the sensitivity of the SSCT to injuries. Two types of surgical interventions were performed in a rabbit model: A skin incision with tendon laceration and SSCT stretching sufficient to damage the SSCT, and skin incision alone. Twelve weeks after surgery, the rabbit carpal tunnel tissues were studied with immunochemistry for TGF-β receptors 1, 2, and 3, collagen III, and collagen VI. All TGF-β receptors were expressed. The percentages of the TGF-β receptors' expressions were less in the control SSCT fibroblasts than in the fibroblasts from rabbits with surgical interventions. The surgical interventions did not result in any alteration of collagen III expression. However, both surgical interventions resulted in a significant decrease in collagen VI expression compared to the control group. The two surgical interventions achieved similar expression of TGF-β receptors and collagens. Our results provide evidence that the SSCT is sensitive to surgical interventions, even when these are modest. Since SSCT fibrosis is a hallmark of CTS, these data also suggest that such fibrosis could result from relatively minor trauma.

Original languageEnglish (US)
Pages (from-to)649-654
Number of pages6
JournalJournal of Orthopaedic Research
Volume30
Issue number4
DOIs
StatePublished - Apr 2012

Fingerprint

Carpal Tunnel Syndrome
Connective Tissue
Rabbits
Fibrosis
Collagen
Wounds and Injuries
Fibroblasts
Collagen Receptors
Immunochemistry
Skin
Lacerations
Wrist
Tendons
Control Groups

Keywords

  • carpal tunnel syndrome
  • subsynovial connective tissue

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

Subsynovial connective tissue is sensitive to surgical interventions in a rabbit model of carpal tunnel syndrome. / Sun, Yu Long; Moriya, Tamami; Zhao, Chunfeng D; Kirk, Ramona L.; Chikenji, Takako; Passe, Sandra M.; An, Kai Nan; Amadio, Peter C.

In: Journal of Orthopaedic Research, Vol. 30, No. 4, 04.2012, p. 649-654.

Research output: Contribution to journalArticle

Sun, Yu Long ; Moriya, Tamami ; Zhao, Chunfeng D ; Kirk, Ramona L. ; Chikenji, Takako ; Passe, Sandra M. ; An, Kai Nan ; Amadio, Peter C. / Subsynovial connective tissue is sensitive to surgical interventions in a rabbit model of carpal tunnel syndrome. In: Journal of Orthopaedic Research. 2012 ; Vol. 30, No. 4. pp. 649-654.
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