Substrate and inhibitor related characteristics of monoamine oxidase in C6 rat glial cells

D. L. Murphy, C. H. Donnelly, E. Richelson

Research output: Contribution to journalArticlepeer-review

52 Scopus citations

Abstract

Cultured C6 rat glial cells preferentially deaminated 5 hydroxytryptamine, tryptamine, dopamine and tyramine in comparison to phenylethylamine and benzylamine. Deamination of all substrates was uniformly sensitive to inhibition by clorgyline and relatively insensitive to deprenyl. These data together with the observations of simple sigmoid curves for the inhibition of tyramine deamination by both inhibitors suggest that C6 glial cells contain mainly monoamine oxidase type A, which previously had been suggested to be primarily an intraneuronal MAO type. As these findings are in agreement with other studies of brain MAO activity in mitochondria separated from neuronal vs glial cell preparations, they help explain why MAO activity measured with some substrates may be little affected by lesions or by drugs producing nerve ending degeneration.

Original languageEnglish (US)
Pages (from-to)1231-1235
Number of pages5
JournalJournal of Neurochemistry
Volume26
Issue number6
StatePublished - 1976
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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