TY - JOUR
T1 - SUBSTRATE AND INHIBITOR‐RELATED CHARACTERISTICS OF MONOAMINE OXIDASE IN C6 RAT GLIAL CELLS
AU - Murphy, D. L.
AU - Donnelly, Cynthia H.
AU - Richelson, E.
PY - 1976/6
Y1 - 1976/6
N2 - Cultured C6 rat glial cells preferentially deaminated 5‐hydroxytryptamine, tryptamine, dopamine and tyramine in comparison to phenylethylamine and benzylamine. Deamination of all substrates was uniformly sensitive to inhibition by clorgyline and relatively insensitive to deprenyl. These data together with the observations of simple sigmoid curves for the inhibition of tyramine deamination by both inhibitors suggest that C6 glial cells contain mainly monoamine oxidase type A, which previously had been suggested to be primarily an intraneuronal MAO type. As these findings are in agreement with other studies of brain MA0 activity in mitochondria separated from neuronal vs glial cell preparations, they help explain why MA0 activity measured with some substrates may be little affected by lesions or by drugs producing nerve ending degeneration.
AB - Cultured C6 rat glial cells preferentially deaminated 5‐hydroxytryptamine, tryptamine, dopamine and tyramine in comparison to phenylethylamine and benzylamine. Deamination of all substrates was uniformly sensitive to inhibition by clorgyline and relatively insensitive to deprenyl. These data together with the observations of simple sigmoid curves for the inhibition of tyramine deamination by both inhibitors suggest that C6 glial cells contain mainly monoamine oxidase type A, which previously had been suggested to be primarily an intraneuronal MAO type. As these findings are in agreement with other studies of brain MA0 activity in mitochondria separated from neuronal vs glial cell preparations, they help explain why MA0 activity measured with some substrates may be little affected by lesions or by drugs producing nerve ending degeneration.
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U2 - 10.1111/j.1471-4159.1976.tb07011.x
DO - 10.1111/j.1471-4159.1976.tb07011.x
M3 - Article
C2 - 932726
AN - SCOPUS:0017136183
SN - 0022-3042
VL - 26
SP - 1231
EP - 1235
JO - Journal of neurochemistry
JF - Journal of neurochemistry
IS - 6
ER -