Substrate adhesion affects contraction and mechanical properties of fibroblast populated collagen lattices

Meng Yi Chen, Yu Long Sun, Chunfeng Zhao, Mark E. Zobitz, Kai Nan An, Steven L. Moran, Peter C. Amadio

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Fibroblasts can condense a hydrated collagen lattice to a tissue-like structure. The purpose of this study was to evaluate the effect of substrate adhesion on the contraction and mechanical properties of fibroblast populated collagen lattices. Bacteriological grade polystyrene (BGPS) plates and tissue culture polystyrene (TCPS) plates were used as substrates for incubation of fibroblast populated collagen lattices. Hydrophobicity of the polystyrene surfaces was measured by the static sessile contact angle method. Collagen lattice contraction was recorded for 2 weeks, after which the lattices were mechanically tested. The BGPS culture plate had a significantly larger contact angle and was more hydrophobic than the TCPS culture plate. Both hydrophobicity and peripheral detachment of the collagen gel significantly decreased the time lag before initiation of gel contraction and increased the strength of the fibroblast populated collagen lattices. Substrate adhesion affects the contractility and strength of cell seeded collagen gels. This information may be useful in developing tissue engineered tendons and ligaments.

Original languageEnglish (US)
Pages (from-to)218-223
Number of pages6
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Volume84
Issue number1
DOIs
StatePublished - Jan 1 2008

Keywords

  • Adhesion force
  • Collagen lattice
  • Fibroblast
  • Substrate

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

Fingerprint Dive into the research topics of 'Substrate adhesion affects contraction and mechanical properties of fibroblast populated collagen lattices'. Together they form a unique fingerprint.

  • Cite this