Substitution of 3′-phosphate cap with a carbon-based blocker reduces the possibility of fluorescence resonance energy transfer probe failure in real-time PCR assays

Kendall W. Cradic; Jason E. Wells; Lindsay Allen; Kent E. Kruckeberg; Ravinder J. Singh; Stefan K.G. Grebe

doi:10.1373/clinchem.2004.033183

Substitution of 3′-phosphate cap with a carbon-based blocker reduces the possibility of fluorescence resonance energy transfer probe failure in real-time PCR assays

Kendall W. Cradic, Jason E. Wells, Lindsay Allen, Kent E. Kruckeberg, Ravinder J. Singh, Stefan K.G. Grebe

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Original language	English (US)
Pages (from-to)	1080-1082
Number of pages	3
Journal	Clinical chemistry
Volume	50
Issue number	6
DOIs	https://doi.org/10.1373/clinchem.2004.033183
State	Published - Jun 2004

ASJC Scopus subject areas

Clinical Biochemistry
Biochemistry, medical

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10.1373/clinchem.2004.033183

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@article{5c60feade95f45ed8f38ae641661263c,

title = "Substitution of 3′-phosphate cap with a carbon-based blocker reduces the possibility of fluorescence resonance energy transfer probe failure in real-time PCR assays",

author = "Cradic, {Kendall W.} and Wells, {Jason E.} and Lindsay Allen and Kruckeberg, {Kent E.} and Singh, {Ravinder J.} and Grebe, {Stefan K.G.}",

year = "2004",

month = jun,

doi = "10.1373/clinchem.2004.033183",

language = "English (US)",

volume = "50",

pages = "1080--1082",

journal = "Clinical Chemistry",

issn = "0009-9147",

publisher = "American Association for Clinical Chemistry Inc.",

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TY - JOUR

T1 - Substitution of 3′-phosphate cap with a carbon-based blocker reduces the possibility of fluorescence resonance energy transfer probe failure in real-time PCR assays

AU - Cradic, Kendall W.

AU - Wells, Jason E.

AU - Allen, Lindsay

AU - Kruckeberg, Kent E.

AU - Singh, Ravinder J.

AU - Grebe, Stefan K.G.

PY - 2004/6

Y1 - 2004/6

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U2 - 10.1373/clinchem.2004.033183

DO - 10.1373/clinchem.2004.033183

M3 - Article

C2 - 15161729

AN - SCOPUS:2642535144

SN - 0009-9147

VL - 50

SP - 1080

EP - 1082

JO - Clinical Chemistry

JF - Clinical Chemistry

IS - 6

ER -

Substitution of 3′-phosphate cap with a carbon-based blocker reduces the possibility of fluorescence resonance energy transfer probe failure in real-time PCR assays

ASJC Scopus subject areas

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