Subnuclear localization and intranuclear trafficking of transcription factors

Sayyed K. Zaidi, Ricardo F. Medina, Shirwin M. Pockwinse, Rachit Bakshi, Krishna P. Kota, Syed A. Ali, Daniel W. Young, Jeffrey A. Nickerson, Amjad Javed, Martin Montecino, Andre J. Van Wijnen, Jane B. Lian, Janet L. Stein, Gary S. Stein

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Nuclear microenvironments are architecturally organized subnuclear sites where the regulatory machinery for gene expression, replication, and repair resides. This compartmentalization is necessary to attain required stoichiometry for organization and assembly of regulatory complexes for combinatorial control. Combined and methodical application of molecular, cellular, biochemical, and in vivo genetic approaches is required to fully understand complexities of biological control. Here we provide methodologies to characterize nuclear organization of regulatory machinery by in situ immunofluorescence microscopy.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages77-93
Number of pages17
Volume647
DOIs
StatePublished - 2010
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume647
ISSN (Print)10643745

Keywords

  • Confocal microscopy
  • FRAP
  • Immunofluorescence microscopy
  • Live cell microscopy
  • Nuclear matrix
  • Nuclear organization
  • Runx

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

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  • Cite this

    Zaidi, S. K., Medina, R. F., Pockwinse, S. M., Bakshi, R., Kota, K. P., Ali, S. A., Young, D. W., Nickerson, J. A., Javed, A., Montecino, M., Van Wijnen, A. J., Lian, J. B., Stein, J. L., & Stein, G. S. (2010). Subnuclear localization and intranuclear trafficking of transcription factors. In Methods in Molecular Biology (Vol. 647, pp. 77-93). (Methods in Molecular Biology; Vol. 647). https://doi.org/10.1007/978-1-60761-738-9-4