Subnuclear localization and intranuclear trafficking of transcription factors

Sayyed K. Zaidi; Ricardo F. Medina; Shirwin M. Pockwinse; Rachit Bakshi; Krishna P. Kota; Syed A. Ali; Daniel W. Young; Jeffrey A. Nickerson; Amjad Javed; Martin Montecino; Andre J. Van Wijnen; Jane B. Lian; Janet L. Stein; Gary S. Stein

doi:10.1007/978-1-60761-738-9-4

Subnuclear localization and intranuclear trafficking of transcription factors

Sayyed K. Zaidi, Ricardo F. Medina, Shirwin M. Pockwinse, Rachit Bakshi, Krishna P. Kota, Syed A. Ali, Daniel W. Young, Jeffrey A. Nickerson, Amjad Javed, Martin Montecino, Andre J. Van Wijnen, Jane B. Lian, Janet L. Stein, Gary S. Stein

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Nuclear microenvironments are architecturally organized subnuclear sites where the regulatory machinery for gene expression, replication, and repair resides. This compartmentalization is necessary to attain required stoichiometry for organization and assembly of regulatory complexes for combinatorial control. Combined and methodical application of molecular, cellular, biochemical, and in vivo genetic approaches is required to fully understand complexities of biological control. Here we provide methodologies to characterize nuclear organization of regulatory machinery by in situ immunofluorescence microscopy.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Pages	77-93
Number of pages	17
Volume	647
DOIs	https://doi.org/10.1007/978-1-60761-738-9-4
State	Published - 2010
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	647
ISSN (Print)	10643745

Keywords

Confocal microscopy
FRAP
Immunofluorescence microscopy
Live cell microscopy
Nuclear matrix
Nuclear organization
Runx

ASJC Scopus subject areas

Molecular Biology
Genetics
Medicine(all)

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10.1007/978-1-60761-738-9-4

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Zaidi, S. K., Medina, R. F., Pockwinse, S. M., Bakshi, R., Kota, K. P., Ali, S. A., Young, D. W., Nickerson, J. A., Javed, A., Montecino, M., Van Wijnen, A. J., Lian, J. B., Stein, J. L., & Stein, G. S. (2010). Subnuclear localization and intranuclear trafficking of transcription factors. In Methods in Molecular Biology (Vol. 647, pp. 77-93). (Methods in Molecular Biology; Vol. 647). https://doi.org/10.1007/978-1-60761-738-9-4

Zaidi, SK, Medina, RF, Pockwinse, SM, Bakshi, R, Kota, KP, Ali, SA, Young, DW, Nickerson, JA, Javed, A, Montecino, M, Van Wijnen, AJ, Lian, JB, Stein, JL & Stein, GS 2010, Subnuclear localization and intranuclear trafficking of transcription factors. in Methods in Molecular Biology. vol. 647, Methods in Molecular Biology, vol. 647, pp. 77-93. https://doi.org/10.1007/978-1-60761-738-9-4

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title = "Subnuclear localization and intranuclear trafficking of transcription factors",

abstract = "Nuclear microenvironments are architecturally organized subnuclear sites where the regulatory machinery for gene expression, replication, and repair resides. This compartmentalization is necessary to attain required stoichiometry for organization and assembly of regulatory complexes for combinatorial control. Combined and methodical application of molecular, cellular, biochemical, and in vivo genetic approaches is required to fully understand complexities of biological control. Here we provide methodologies to characterize nuclear organization of regulatory machinery by in situ immunofluorescence microscopy.",

keywords = "Confocal microscopy, FRAP, Immunofluorescence microscopy, Live cell microscopy, Nuclear matrix, Nuclear organization, Runx",

author = "Zaidi, {Sayyed K.} and Medina, {Ricardo F.} and Pockwinse, {Shirwin M.} and Rachit Bakshi and Kota, {Krishna P.} and Ali, {Syed A.} and Young, {Daniel W.} and Nickerson, {Jeffrey A.} and Amjad Javed and Martin Montecino and {Van Wijnen}, {Andre J.} and Lian, {Jane B.} and Stein, {Janet L.} and Stein, {Gary S.}",

year = "2010",

doi = "10.1007/978-1-60761-738-9-4",

language = "English (US)",

isbn = "9781607617372",

volume = "647",

series = "Methods in Molecular Biology",

pages = "77--93",

booktitle = "Methods in Molecular Biology",

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T1 - Subnuclear localization and intranuclear trafficking of transcription factors

AU - Zaidi, Sayyed K.

AU - Medina, Ricardo F.

AU - Pockwinse, Shirwin M.

AU - Bakshi, Rachit

AU - Kota, Krishna P.

AU - Ali, Syed A.

AU - Young, Daniel W.

AU - Nickerson, Jeffrey A.

AU - Javed, Amjad

AU - Montecino, Martin

AU - Van Wijnen, Andre J.

AU - Lian, Jane B.

AU - Stein, Janet L.

AU - Stein, Gary S.

PY - 2010

Y1 - 2010

N2 - Nuclear microenvironments are architecturally organized subnuclear sites where the regulatory machinery for gene expression, replication, and repair resides. This compartmentalization is necessary to attain required stoichiometry for organization and assembly of regulatory complexes for combinatorial control. Combined and methodical application of molecular, cellular, biochemical, and in vivo genetic approaches is required to fully understand complexities of biological control. Here we provide methodologies to characterize nuclear organization of regulatory machinery by in situ immunofluorescence microscopy.

AB - Nuclear microenvironments are architecturally organized subnuclear sites where the regulatory machinery for gene expression, replication, and repair resides. This compartmentalization is necessary to attain required stoichiometry for organization and assembly of regulatory complexes for combinatorial control. Combined and methodical application of molecular, cellular, biochemical, and in vivo genetic approaches is required to fully understand complexities of biological control. Here we provide methodologies to characterize nuclear organization of regulatory machinery by in situ immunofluorescence microscopy.

KW - Confocal microscopy

KW - FRAP

KW - Immunofluorescence microscopy

KW - Live cell microscopy

KW - Nuclear matrix

KW - Nuclear organization

KW - Runx

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DO - 10.1007/978-1-60761-738-9-4

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SN - 9781607617372

VL - 647

T3 - Methods in Molecular Biology

SP - 77

EP - 93

BT - Methods in Molecular Biology

ER -

Subnuclear localization and intranuclear trafficking of transcription factors

Abstract

Publication series

Keywords

ASJC Scopus subject areas

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