@inbook{939e05c1855647ae9cd31b07de2ae3cc,
title = "Subnuclear localization and intranuclear trafficking of transcription factors",
abstract = "Nuclear microenvironments are architecturally organized subnuclear sites where the regulatory machinery for gene expression, replication, and repair resides. This compartmentalization is necessary to attain required stoichiometry for organization and assembly of regulatory complexes for combinatorial control. Combined and methodical application of molecular, cellular, biochemical, and in vivo genetic approaches is required to fully understand complexities of biological control. Here we provide methodologies to characterize nuclear organization of regulatory machinery by in situ immunofluorescence microscopy.",
keywords = "Confocal microscopy, FRAP, Immunofluorescence microscopy, Live cell microscopy, Nuclear matrix, Nuclear organization, Runx",
author = "Zaidi, {Sayyed K.} and Medina, {Ricardo F.} and Pockwinse, {Shirwin M.} and Rachit Bakshi and Kota, {Krishna P.} and Ali, {Syed A.} and Young, {Daniel W.} and Nickerson, {Jeffrey A.} and Amjad Javed and Martin Montecino and {Van Wijnen}, {Andre J.} and Lian, {Jane B.} and Stein, {Janet L.} and Stein, {Gary S.}",
year = "2010",
doi = "10.1007/978-1-60761-738-9_4",
language = "English (US)",
isbn = "9781607617372",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "77--93",
booktitle = "Transcription Factors",
}