Subnuclear compartmentalization of immunoglobulin loci during lymphocyte development

Steven T. Kosak, Jane A. Skok, Kay L Medina, Roy Riblet, Michelle M. Le Beau, Amanda G. Fisher, Harinder Singh

Research output: Contribution to journalArticle

525 Citations (Scopus)

Abstract

Immunoglobulin (Ig) loci are selectively activated for transcription and rearrangement during B lymphocyte development. Using fluorescence in situ hybridization, we show that Ig heavy (H) and Igκ loci are preferentially positioned at the nuclear periphery in hematopoietic progenitors and pro-T cells but are centrally configured in pro-B nuclei. The inactive loci at the periphery do not associate with centromeric heterochromatin. Upon localization away from the nuclear periphery in pro-B cells, the IgH locus appears to undergo large-scale compaction. We suggest that subnuclear positioning represents a novel means of regulating transcription and recombination of IgH and Igκ loci during lymphocyte development.

Original languageEnglish (US)
Pages (from-to)158-162
Number of pages5
JournalScience
Volume296
Issue number5565
DOIs
StatePublished - Apr 5 2002
Externally publishedYes

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Immunoglobulins
Lymphocytes
B-Lymphoid Precursor Cells
Heterochromatin
Fluorescence In Situ Hybridization
Genetic Recombination
B-Lymphocytes
T-Lymphocytes

ASJC Scopus subject areas

  • General

Cite this

Kosak, S. T., Skok, J. A., Medina, K. L., Riblet, R., Le Beau, M. M., Fisher, A. G., & Singh, H. (2002). Subnuclear compartmentalization of immunoglobulin loci during lymphocyte development. Science, 296(5565), 158-162. https://doi.org/10.1126/science.1068768

Subnuclear compartmentalization of immunoglobulin loci during lymphocyte development. / Kosak, Steven T.; Skok, Jane A.; Medina, Kay L; Riblet, Roy; Le Beau, Michelle M.; Fisher, Amanda G.; Singh, Harinder.

In: Science, Vol. 296, No. 5565, 05.04.2002, p. 158-162.

Research output: Contribution to journalArticle

Kosak, ST, Skok, JA, Medina, KL, Riblet, R, Le Beau, MM, Fisher, AG & Singh, H 2002, 'Subnuclear compartmentalization of immunoglobulin loci during lymphocyte development', Science, vol. 296, no. 5565, pp. 158-162. https://doi.org/10.1126/science.1068768
Kosak, Steven T. ; Skok, Jane A. ; Medina, Kay L ; Riblet, Roy ; Le Beau, Michelle M. ; Fisher, Amanda G. ; Singh, Harinder. / Subnuclear compartmentalization of immunoglobulin loci during lymphocyte development. In: Science. 2002 ; Vol. 296, No. 5565. pp. 158-162.
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