Subnuclear compartmentalization of immunoglobulin loci during lymphocyte development

Steven T. Kosak, Jane A. Skok, Kay L. Medina, Roy Riblet, Michelle M. Le Beau, Amanda G. Fisher, Harinder Singh

Research output: Contribution to journalArticlepeer-review

547 Scopus citations

Abstract

Immunoglobulin (Ig) loci are selectively activated for transcription and rearrangement during B lymphocyte development. Using fluorescence in situ hybridization, we show that Ig heavy (H) and Igκ loci are preferentially positioned at the nuclear periphery in hematopoietic progenitors and pro-T cells but are centrally configured in pro-B nuclei. The inactive loci at the periphery do not associate with centromeric heterochromatin. Upon localization away from the nuclear periphery in pro-B cells, the IgH locus appears to undergo large-scale compaction. We suggest that subnuclear positioning represents a novel means of regulating transcription and recombination of IgH and Igκ loci during lymphocyte development.

Original languageEnglish (US)
Pages (from-to)158-162
Number of pages5
JournalScience
Volume296
Issue number5565
DOIs
StatePublished - Apr 5 2002

ASJC Scopus subject areas

  • General

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