Subnuclear compartmentalization of immunoglobulin loci during lymphocyte development

Steven T. Kosak, Jane A. Skok, Kay L. Medina, Roy Riblet, Michelle M. Le Beau, Amanda G. Fisher, Harinder Singh

Research output: Contribution to journalArticle

539 Scopus citations

Abstract

Immunoglobulin (Ig) loci are selectively activated for transcription and rearrangement during B lymphocyte development. Using fluorescence in situ hybridization, we show that Ig heavy (H) and Igκ loci are preferentially positioned at the nuclear periphery in hematopoietic progenitors and pro-T cells but are centrally configured in pro-B nuclei. The inactive loci at the periphery do not associate with centromeric heterochromatin. Upon localization away from the nuclear periphery in pro-B cells, the IgH locus appears to undergo large-scale compaction. We suggest that subnuclear positioning represents a novel means of regulating transcription and recombination of IgH and Igκ loci during lymphocyte development.

Original languageEnglish (US)
Pages (from-to)158-162
Number of pages5
JournalScience
Volume296
Issue number5565
DOIs
StatePublished - Apr 5 2002

ASJC Scopus subject areas

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    Kosak, S. T., Skok, J. A., Medina, K. L., Riblet, R., Le Beau, M. M., Fisher, A. G., & Singh, H. (2002). Subnuclear compartmentalization of immunoglobulin loci during lymphocyte development. Science, 296(5565), 158-162. https://doi.org/10.1126/science.1068768