Subcutaneous adipose tissue releases interleukin-6, but not tumor necrosis factor-α, in vivo

V. Mohamed-Ali, S. Goodrick, A. Rawesh, D. R. Katz, J. M. Miles, J. S. Yudkin, S. Klein, S. W. Coppack

Research output: Contribution to journalArticle

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Abstract

We measured arterio-venous differences in concentrations of tumor necrosis facter-α (TNFα) and interleukin-6 (IL-6) across a sc adipose tissue bed in the postabsorptive state in 39 subjects [22 women and 17 men; median age, 36 yr (interquartile range, 26 - 48 yr); body mass index, 31.8 kg/m2 (range, 22.3- 38.7 kg/m2); percent body fat, 28.7% (range, 17.6- 60.7%)]. A subgroup of 8 subjects had arteriovenous differences measured across forearm muscle. Thirty subjects were studied from late morning to early evening; 19 ate a high carbohydrate meal around 1300 h, and 11 continued to fast. We found a greater than 2-fold increase in IL-6 concentrations across the adipose tissue bed [arterial, 2.27 pg/mL (range, 1.42-3.53 pg/mL); venous, 6.71 pg/mL (range, 3.36-9.62 pg/mL); P < 0.001], but not across forearm muscle. Arterial plasma concentrations of IL-6 correlated significantly with body mass index (Spearman's r = 0.48; P < 0.01) and percent body fat (Spearman's r = 0.49; P < 0.01). Subcutaneous adipose tissue IL-6 production increased by the early evening (1800-1900 h) in both subjects who had extended their fasting and those who had eaten. Neither deep forearm nor sc adipose tissue consistently released TNFα (across adipose tissue: arterial, 1.83 pg/mL (range, 1.36-2.34 pg/mL); venous, 1.85 pg/mL (range, 1.44-2.53 pg/mL); P = NS: across forearm muscle: arterial, 1.22 pg/mL (range, 0,74-2.76 pg/mL); venous, 0.99 pg/mL (range, 0.69-1.70 pg/mL); P = NS]. Al though beth IL-6 and TNFα are expressed by adipose tissue, our results show that there are important differences in their systemic release. TNFα is not released by this sc depot. In contrast, IL-6 is released from the depot and is thereby able to signal systemically.

Original languageEnglish (US)
Pages (from-to)4196-4200
Number of pages5
JournalJournal of Clinical Endocrinology and Metabolism
Volume82
Issue number12
StatePublished - 1997
Externally publishedYes

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Subcutaneous Fat
Adipose Tissue
Interleukin-6
Tumor Necrosis Factor-alpha
Tissue
Tumors
Forearm
Necrosis
Muscle
Muscles
Fats
Neoplasms
Body Mass Index
Meals
Carbohydrates
Fasting
Plasmas

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Mohamed-Ali, V., Goodrick, S., Rawesh, A., Katz, D. R., Miles, J. M., Yudkin, J. S., ... Coppack, S. W. (1997). Subcutaneous adipose tissue releases interleukin-6, but not tumor necrosis factor-α, in vivo. Journal of Clinical Endocrinology and Metabolism, 82(12), 4196-4200.

Subcutaneous adipose tissue releases interleukin-6, but not tumor necrosis factor-α, in vivo. / Mohamed-Ali, V.; Goodrick, S.; Rawesh, A.; Katz, D. R.; Miles, J. M.; Yudkin, J. S.; Klein, S.; Coppack, S. W.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 82, No. 12, 1997, p. 4196-4200.

Research output: Contribution to journalArticle

Mohamed-Ali, V, Goodrick, S, Rawesh, A, Katz, DR, Miles, JM, Yudkin, JS, Klein, S & Coppack, SW 1997, 'Subcutaneous adipose tissue releases interleukin-6, but not tumor necrosis factor-α, in vivo', Journal of Clinical Endocrinology and Metabolism, vol. 82, no. 12, pp. 4196-4200.
Mohamed-Ali V, Goodrick S, Rawesh A, Katz DR, Miles JM, Yudkin JS et al. Subcutaneous adipose tissue releases interleukin-6, but not tumor necrosis factor-α, in vivo. Journal of Clinical Endocrinology and Metabolism. 1997;82(12):4196-4200.
Mohamed-Ali, V. ; Goodrick, S. ; Rawesh, A. ; Katz, D. R. ; Miles, J. M. ; Yudkin, J. S. ; Klein, S. ; Coppack, S. W. / Subcutaneous adipose tissue releases interleukin-6, but not tumor necrosis factor-α, in vivo. In: Journal of Clinical Endocrinology and Metabolism. 1997 ; Vol. 82, No. 12. pp. 4196-4200.
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AU - Rawesh, A.

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AU - Miles, J. M.

AU - Yudkin, J. S.

AU - Klein, S.

AU - Coppack, S. W.

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N2 - We measured arterio-venous differences in concentrations of tumor necrosis facter-α (TNFα) and interleukin-6 (IL-6) across a sc adipose tissue bed in the postabsorptive state in 39 subjects [22 women and 17 men; median age, 36 yr (interquartile range, 26 - 48 yr); body mass index, 31.8 kg/m2 (range, 22.3- 38.7 kg/m2); percent body fat, 28.7% (range, 17.6- 60.7%)]. A subgroup of 8 subjects had arteriovenous differences measured across forearm muscle. Thirty subjects were studied from late morning to early evening; 19 ate a high carbohydrate meal around 1300 h, and 11 continued to fast. We found a greater than 2-fold increase in IL-6 concentrations across the adipose tissue bed [arterial, 2.27 pg/mL (range, 1.42-3.53 pg/mL); venous, 6.71 pg/mL (range, 3.36-9.62 pg/mL); P < 0.001], but not across forearm muscle. Arterial plasma concentrations of IL-6 correlated significantly with body mass index (Spearman's r = 0.48; P < 0.01) and percent body fat (Spearman's r = 0.49; P < 0.01). Subcutaneous adipose tissue IL-6 production increased by the early evening (1800-1900 h) in both subjects who had extended their fasting and those who had eaten. Neither deep forearm nor sc adipose tissue consistently released TNFα (across adipose tissue: arterial, 1.83 pg/mL (range, 1.36-2.34 pg/mL); venous, 1.85 pg/mL (range, 1.44-2.53 pg/mL); P = NS: across forearm muscle: arterial, 1.22 pg/mL (range, 0,74-2.76 pg/mL); venous, 0.99 pg/mL (range, 0.69-1.70 pg/mL); P = NS]. Al though beth IL-6 and TNFα are expressed by adipose tissue, our results show that there are important differences in their systemic release. TNFα is not released by this sc depot. In contrast, IL-6 is released from the depot and is thereby able to signal systemically.

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