TY - JOUR
T1 - Studies to improve fixation of human nerves. Part 2. Effect of time elapsed between death and glutaraldehyde fixation on relationship of axonal area to number of myelin lamellae
AU - Ohnishi, Akio
AU - O'Brien, Peter C.
AU - Dyck, Peter James
N1 - Funding Information:
This investigation was supported in part by Research Grants NS-5811, NS-7541, and 72-2200 from the National Institutes of Health, Public Health Service, and by grants from the Miller, Gallmeyer, Upton, and Heller Funds.
PY - 1974/11
Y1 - 1974/11
N2 - Glutaraldehyde fixation after death of the animal (simulated autopsy) was associated with a statistically significant change of the relationship between transverse area of axis cylinder and number of myelin lamellae from that in the controls (simulated biopsy). Differences in length of time elapsed prior to glutaraldehyde fixation (1, 6, or 12 hr) had no significant effect. When the fixation was begun after death, in fibers with more than 72 myelin lamellae the area of axis cylinder was smaller, but in fibers with less than 71 myelin lamellae it was larger than in controls.
AB - Glutaraldehyde fixation after death of the animal (simulated autopsy) was associated with a statistically significant change of the relationship between transverse area of axis cylinder and number of myelin lamellae from that in the controls (simulated biopsy). Differences in length of time elapsed prior to glutaraldehyde fixation (1, 6, or 12 hr) had no significant effect. When the fixation was begun after death, in fibers with more than 72 myelin lamellae the area of axis cylinder was smaller, but in fibers with less than 71 myelin lamellae it was larger than in controls.
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U2 - 10.1016/0022-510X(74)90156-7
DO - 10.1016/0022-510X(74)90156-7
M3 - Article
C2 - 4214901
AN - SCOPUS:0016203618
SN - 0022-510X
VL - 23
SP - 387
EP - 390
JO - Journal of the neurological sciences
JF - Journal of the neurological sciences
IS - 3
ER -