Studies to improve fixation of human nerves. Part 1. Effect of duration of glutaraldehyde fixation on peripheral nerve morphometry

A. Ohnishi, K. Offord, Peter J Dyck

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Abstract

No change in the transverse fascicular area of rat peroneal nerve occurred when the duration of fixation in 2% glutaraldehyde was increased frm 1 to 12 hr. Shrinkage in endoneurial area was inferred from the development of clefts. The number of myelin lamellae remained constant when the nerve was fixed in glutaraldehyde for 1 or for 12 hr. Unequivocal shrinkage of axis cylinders occurred with prolongation of glutaraldehyde fixation beyond 1 hr. In nerves fixed for 12 hr, this shrinkage was approximately 20%, compared to the area at 1 hr.

Original languageEnglish (US)
Pages (from-to)223-226
Number of pages4
JournalJournal of the Neurological Sciences
Volume23
Issue number2
DOIs
StatePublished - 1974

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Glutaral
Peripheral Nerves
Peroneal Nerve
Myelin Sheath

ASJC Scopus subject areas

  • Aging
  • Clinical Neurology
  • Surgery
  • Neuroscience(all)
  • Developmental Neuroscience
  • Neurology

Cite this

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AB - No change in the transverse fascicular area of rat peroneal nerve occurred when the duration of fixation in 2% glutaraldehyde was increased frm 1 to 12 hr. Shrinkage in endoneurial area was inferred from the development of clefts. The number of myelin lamellae remained constant when the nerve was fixed in glutaraldehyde for 1 or for 12 hr. Unequivocal shrinkage of axis cylinders occurred with prolongation of glutaraldehyde fixation beyond 1 hr. In nerves fixed for 12 hr, this shrinkage was approximately 20%, compared to the area at 1 hr.

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