Studies to improve fixation of human nerves. Part 1. Effect of duration of glutaraldehyde fixation on peripheral nerve morphometry

Akio Ohnishi; Kenneth Offord; Peter James Dyck

doi:10.1016/0022-510X(74)90225-1

Studies to improve fixation of human nerves. Part 1. Effect of duration of glutaraldehyde fixation on peripheral nerve morphometry

Akio Ohnishi, Kenneth Offord, Peter James Dyck

Neurology

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

No change in the transverse fascicular area of rat peroneal nerve occurred when the duration of fixation in 2% glutaraldehyde was increased from 1 to 12 hr. Shrinkage in endoneurial area was inferred from the development of clefts. The number of myelin lamellae remained constant when the nerve was fixed in glutaraldehyde for 1 or for 12 hr. Unequivocal shrinkage of axis cylinders occurred with prolongation of glutaraldehyde fixation beyond 1 hr. In nerves fixed for 12 hr, this shrinkage was approximately 20%, compared to the area at 1 hr.

Original language	English (US)
Pages (from-to)	223-226
Number of pages	4
Journal	Journal of the neurological sciences
Volume	23
Issue number	2
DOIs	https://doi.org/10.1016/0022-510X(74)90225-1
State	Published - Oct 1974

ASJC Scopus subject areas

Neurology
Clinical Neurology

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10.1016/0022-510X(74)90225-1

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title = "Studies to improve fixation of human nerves. Part 1. Effect of duration of glutaraldehyde fixation on peripheral nerve morphometry",

abstract = "No change in the transverse fascicular area of rat peroneal nerve occurred when the duration of fixation in 2% glutaraldehyde was increased from 1 to 12 hr. Shrinkage in endoneurial area was inferred from the development of clefts. The number of myelin lamellae remained constant when the nerve was fixed in glutaraldehyde for 1 or for 12 hr. Unequivocal shrinkage of axis cylinders occurred with prolongation of glutaraldehyde fixation beyond 1 hr. In nerves fixed for 12 hr, this shrinkage was approximately 20%, compared to the area at 1 hr.",

author = "Akio Ohnishi and Kenneth Offord and Dyck, {Peter James}",

note = "Funding Information: Transverse fascicular area was measured on photographic enlargements of 1.5-ktm-thick transverse sections. The inner boundary of the perineurium was traced with the This investigation was supported in part by Research Grants NS-5811, NS-7541, and 72-2200 from the National Institutes of Health, Public Health Service,a nd by grants from the Miller, Gallmeyer,a nd Upton Funds.",

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AU - Offord, Kenneth

AU - Dyck, Peter James

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PY - 1974/10

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N2 - No change in the transverse fascicular area of rat peroneal nerve occurred when the duration of fixation in 2% glutaraldehyde was increased from 1 to 12 hr. Shrinkage in endoneurial area was inferred from the development of clefts. The number of myelin lamellae remained constant when the nerve was fixed in glutaraldehyde for 1 or for 12 hr. Unequivocal shrinkage of axis cylinders occurred with prolongation of glutaraldehyde fixation beyond 1 hr. In nerves fixed for 12 hr, this shrinkage was approximately 20%, compared to the area at 1 hr.

AB - No change in the transverse fascicular area of rat peroneal nerve occurred when the duration of fixation in 2% glutaraldehyde was increased from 1 to 12 hr. Shrinkage in endoneurial area was inferred from the development of clefts. The number of myelin lamellae remained constant when the nerve was fixed in glutaraldehyde for 1 or for 12 hr. Unequivocal shrinkage of axis cylinders occurred with prolongation of glutaraldehyde fixation beyond 1 hr. In nerves fixed for 12 hr, this shrinkage was approximately 20%, compared to the area at 1 hr.

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Studies to improve fixation of human nerves. Part 1. Effect of duration of glutaraldehyde fixation on peripheral nerve morphometry

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