Studies on the transport of L tyrosine into an adrenergic clone of mouse neuroblastoma

E. Richelson

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Studies on the transport of L-[U 14C]tyrosine into cultured mouse neuroblastoma clone N1E 115, which has high levels of tyrosine hydroxylase (EC 1.14.16.2), are presented. This transport was by a saturable process, exhibited marked exchanging properties, and was inhibited by a reagent which attacks sulfhydryl groups but not by metabolic inhibitors. No major ion requirements were found for this transport, which was sensitive to changes in temperature but not to changes in pH in the range of 6 to 8. Molecules with close structural analogy to L tyrosine such as L 3,4 dihydroxyphenylalanine, L 3 iodotyrosine, and L phenylalanine were potent competitive inhibitors of this transport with inhibitor constants (Ki) in the range of 2 to 6 x 10-5 M. In addition, compounds such as L isoleucine, L leucine, L methionine, and L valine caused inhibition of L tyrosine transport. These results are consistent with the conclusion that L tyrosine transport into this clone is by facilitated diffusion and by a mechanism similar to the leucine preferring system which has been described by others.

Original languageEnglish (US)
Pages (from-to)6218-6224
Number of pages7
JournalJournal of Biological Chemistry
Volume249
Issue number19
StatePublished - 1974
Externally publishedYes

Fingerprint

Neuroblastoma
Adrenergic Agents
Tyrosine
Clone Cells
Leucine
Facilitated Diffusion
Isoleucine
Valine
Tyrosine 3-Monooxygenase
Levodopa
Phenylalanine
Methionine
Ions
Molecules
Temperature

ASJC Scopus subject areas

  • Biochemistry

Cite this

Studies on the transport of L tyrosine into an adrenergic clone of mouse neuroblastoma. / Richelson, E.

In: Journal of Biological Chemistry, Vol. 249, No. 19, 1974, p. 6218-6224.

Research output: Contribution to journalArticle

@article{2e465979956b4cc1a33d48b2040e1f48,
title = "Studies on the transport of L tyrosine into an adrenergic clone of mouse neuroblastoma",
abstract = "Studies on the transport of L-[U 14C]tyrosine into cultured mouse neuroblastoma clone N1E 115, which has high levels of tyrosine hydroxylase (EC 1.14.16.2), are presented. This transport was by a saturable process, exhibited marked exchanging properties, and was inhibited by a reagent which attacks sulfhydryl groups but not by metabolic inhibitors. No major ion requirements were found for this transport, which was sensitive to changes in temperature but not to changes in pH in the range of 6 to 8. Molecules with close structural analogy to L tyrosine such as L 3,4 dihydroxyphenylalanine, L 3 iodotyrosine, and L phenylalanine were potent competitive inhibitors of this transport with inhibitor constants (Ki) in the range of 2 to 6 x 10-5 M. In addition, compounds such as L isoleucine, L leucine, L methionine, and L valine caused inhibition of L tyrosine transport. These results are consistent with the conclusion that L tyrosine transport into this clone is by facilitated diffusion and by a mechanism similar to the leucine preferring system which has been described by others.",
author = "E. Richelson",
year = "1974",
language = "English (US)",
volume = "249",
pages = "6218--6224",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "19",

}

TY - JOUR

T1 - Studies on the transport of L tyrosine into an adrenergic clone of mouse neuroblastoma

AU - Richelson, E.

PY - 1974

Y1 - 1974

N2 - Studies on the transport of L-[U 14C]tyrosine into cultured mouse neuroblastoma clone N1E 115, which has high levels of tyrosine hydroxylase (EC 1.14.16.2), are presented. This transport was by a saturable process, exhibited marked exchanging properties, and was inhibited by a reagent which attacks sulfhydryl groups but not by metabolic inhibitors. No major ion requirements were found for this transport, which was sensitive to changes in temperature but not to changes in pH in the range of 6 to 8. Molecules with close structural analogy to L tyrosine such as L 3,4 dihydroxyphenylalanine, L 3 iodotyrosine, and L phenylalanine were potent competitive inhibitors of this transport with inhibitor constants (Ki) in the range of 2 to 6 x 10-5 M. In addition, compounds such as L isoleucine, L leucine, L methionine, and L valine caused inhibition of L tyrosine transport. These results are consistent with the conclusion that L tyrosine transport into this clone is by facilitated diffusion and by a mechanism similar to the leucine preferring system which has been described by others.

AB - Studies on the transport of L-[U 14C]tyrosine into cultured mouse neuroblastoma clone N1E 115, which has high levels of tyrosine hydroxylase (EC 1.14.16.2), are presented. This transport was by a saturable process, exhibited marked exchanging properties, and was inhibited by a reagent which attacks sulfhydryl groups but not by metabolic inhibitors. No major ion requirements were found for this transport, which was sensitive to changes in temperature but not to changes in pH in the range of 6 to 8. Molecules with close structural analogy to L tyrosine such as L 3,4 dihydroxyphenylalanine, L 3 iodotyrosine, and L phenylalanine were potent competitive inhibitors of this transport with inhibitor constants (Ki) in the range of 2 to 6 x 10-5 M. In addition, compounds such as L isoleucine, L leucine, L methionine, and L valine caused inhibition of L tyrosine transport. These results are consistent with the conclusion that L tyrosine transport into this clone is by facilitated diffusion and by a mechanism similar to the leucine preferring system which has been described by others.

UR - http://www.scopus.com/inward/record.url?scp=0016201015&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0016201015&partnerID=8YFLogxK

M3 - Article

C2 - 4153813

AN - SCOPUS:0016201015

VL - 249

SP - 6218

EP - 6224

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 19

ER -