1. 1. Co(II) ions increase the Vmax of clostridiopeptidase A, producing a maximum stimulation of overall enzymic activity of 120%. 2. 2. Co(II) does not displace Zn(II) from the active site, nor Ca(II) from its binding site on the enzyme. 3. 3. There appears to be an additional transition metal-binding site on clostridiopeptidase A, accepting Zn(II), which is inhibitory (Ki= 550 μM), or Co(II), which is stimulatory (Kact = 200 μM).
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