TY - JOUR
T1 - Studies on the stimulation of the bacterial, collagenolytic enzyme clostridiopeptidase a by cobalt (ii) ions
AU - Evans, Christopher H.
AU - Mason, Gregg C.
PY - 1986
Y1 - 1986
N2 - 1. 1. Co(II) ions increase the Vmax of clostridiopeptidase A, producing a maximum stimulation of overall enzymic activity of 120%. 2. 2. Co(II) does not displace Zn(II) from the active site, nor Ca(II) from its binding site on the enzyme. 3. 3. There appears to be an additional transition metal-binding site on clostridiopeptidase A, accepting Zn(II), which is inhibitory (Ki= 550 μM), or Co(II), which is stimulatory (Kact = 200 μM).
AB - 1. 1. Co(II) ions increase the Vmax of clostridiopeptidase A, producing a maximum stimulation of overall enzymic activity of 120%. 2. 2. Co(II) does not displace Zn(II) from the active site, nor Ca(II) from its binding site on the enzyme. 3. 3. There appears to be an additional transition metal-binding site on clostridiopeptidase A, accepting Zn(II), which is inhibitory (Ki= 550 μM), or Co(II), which is stimulatory (Kact = 200 μM).
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U2 - 10.1016/0020-711X(86)90014-5
DO - 10.1016/0020-711X(86)90014-5
M3 - Article
C2 - 3002884
AN - SCOPUS:0022643127
SN - 0020-711X
VL - 18
SP - 89
EP - 92
JO - International Journal of Biochemistry
JF - International Journal of Biochemistry
IS - 1
ER -