Structures of human acetylcholinesterase bound to dihydrotanshinone i and territrem B show peripheral site flexibility

Jonah Cheung, Ebony N. Gary, Kazuro Shiomi, Terrone L. Rosenberry

Research output: Contribution to journalArticle

63 Scopus citations


Acetylcholinesterase is a critical enzyme that regulates neurotransmission by degrading the neurotransmitter acetylcholine in synapses of the nervous system. It is an important target for both therapeutic drugs that treat Alzheimer's disease and chemical warfare agents that cripple the nervous system and cause death through paralysis. The enzyme has both catalytic and peripheral sites to which inhibitors may bind. Structures of recombinant human acetylcholinesterase in complex with the natural product inhibitors dihydrotanshinone I and territrem B reveal dihydrotanshinone I binding that is specific to only the peripheral site and territrem B binding that spans both sites and distorts the protein backbone in the peripheral site. These inhibitors may function as important molecular templates for therapeutics used for treatment of disease and protection against nerve agents.

Original languageEnglish (US)
Pages (from-to)1091-1096
Number of pages6
JournalACS Medicinal Chemistry Letters
Issue number11
StatePublished - Nov 14 2013



  • Acetylcholinesterase
  • catalytic site
  • conformational change
  • dihydrotanshinone I
  • peripheral site
  • territrem B

ASJC Scopus subject areas

  • Biochemistry
  • Drug Discovery
  • Organic Chemistry

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