Structure of the ubiquitin hydrolase UCH-L3 complexed with a suicide substrate

Shahram Misaghi, Paul J. Galardy, Wim J N Meester, Huib Ovaa, Hidde L. Ploegh, Rachelle Gaudet

Research output: Contribution to journalArticle

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Abstract

Ubiquitin C-terminal hydrolases (UCHs) comprise a family of small ubiquitin-specific proteases of uncertain function. Although no cellular substrates have been identified for UCHs, their highly tissue-specific expression patterns and the association of UCH-L1 mutations with human disease strongly suggest a critical role. The structure of the yeast UCH Yuh1-ubiquitin aldehyde complex identified an active site crossover loop predicted to limit the size of suitable substrates. We report the 1.45 Å resolution crystal structure of human UCH-L3 in complex with the inhibitor ubiquitin vinylmethylester, an inhibitor that forms a covalent adduct with the active site cysteine of ubiquitin-specific proteases. This structure confirms the predicted mechanism of the inhibitor and allows the direct comparison of a UCH family enzyme in the free and ligand-bound state. We also show the efficient hydrolysis by human UCH-L3 of a 13-residue peptide in isopeptide linkage with ubiquitin, consistent with considerable flexibility in UCH substrate size. We propose a model for the catalytic cycle of UCH family members which accounts for the hydrolysis of larger ubiquitin conjugates.

Original languageEnglish (US)
Pages (from-to)1512-1520
Number of pages9
JournalJournal of Biological Chemistry
Volume280
Issue number2
DOIs
StatePublished - Jan 14 2005
Externally publishedYes

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Ubiquitin Thiolesterase
Hydrolases
Ubiquitin
Suicide
Substrates
Ubiquitin-Specific Proteases
Hydrolysis
Catalytic Domain
hydrolase C
Yeast
Cysteine
Yeasts
Crystal structure

ASJC Scopus subject areas

  • Biochemistry

Cite this

Misaghi, S., Galardy, P. J., Meester, W. J. N., Ovaa, H., Ploegh, H. L., & Gaudet, R. (2005). Structure of the ubiquitin hydrolase UCH-L3 complexed with a suicide substrate. Journal of Biological Chemistry, 280(2), 1512-1520. https://doi.org/10.1074/jbc.M410770200

Structure of the ubiquitin hydrolase UCH-L3 complexed with a suicide substrate. / Misaghi, Shahram; Galardy, Paul J.; Meester, Wim J N; Ovaa, Huib; Ploegh, Hidde L.; Gaudet, Rachelle.

In: Journal of Biological Chemistry, Vol. 280, No. 2, 14.01.2005, p. 1512-1520.

Research output: Contribution to journalArticle

Misaghi, S, Galardy, PJ, Meester, WJN, Ovaa, H, Ploegh, HL & Gaudet, R 2005, 'Structure of the ubiquitin hydrolase UCH-L3 complexed with a suicide substrate', Journal of Biological Chemistry, vol. 280, no. 2, pp. 1512-1520. https://doi.org/10.1074/jbc.M410770200
Misaghi S, Galardy PJ, Meester WJN, Ovaa H, Ploegh HL, Gaudet R. Structure of the ubiquitin hydrolase UCH-L3 complexed with a suicide substrate. Journal of Biological Chemistry. 2005 Jan 14;280(2):1512-1520. https://doi.org/10.1074/jbc.M410770200
Misaghi, Shahram ; Galardy, Paul J. ; Meester, Wim J N ; Ovaa, Huib ; Ploegh, Hidde L. ; Gaudet, Rachelle. / Structure of the ubiquitin hydrolase UCH-L3 complexed with a suicide substrate. In: Journal of Biological Chemistry. 2005 ; Vol. 280, No. 2. pp. 1512-1520.
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