Structure of the rat collagen IV promoter

Joseph P. Grande; Deborah C. Melder; Daniel L. Kluge; Eric D. Wieben

doi:10.1016/S0167-4781(96)00134-0

Structure of the rat collagen IV promoter

Joseph P. Grande, Deborah C. Melder, Daniel L. Kluge, Eric D. Wieben

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

We have isolated a 1.6 kb clone from a rat genomic library which contains the bidirectional collagen IV promoter, flanked by exons coding for the α1(IV) and α2(IV) collagen chains. There are at least two transcription start sites within both the α1(IV) and α2(IV)) collagen genes. Rat mesangial cells were transfected with chloramphenicol acetyltransferase (CAT) reporter plasmids containing segments of the promoter and 5' flanking region, in both the α1(IV) and α2(IV) orientations. Our results suggest that transcriptional efficiency of the bidirectional promoter is more efficient in the α2(IV) direction than in the α1(IV) direction.

Original language	English (US)
Pages (from-to)	85-88
Number of pages	4
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1309
Issue number	1-2
DOIs	https://doi.org/10.1016/S0167-4781(96)00134-0
State	Published - Nov 11 1996

Keywords

Basement membrane
Collagen IV
Promoter
Structure

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

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10.1016/S0167-4781(96)00134-0

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title = "Structure of the rat collagen IV promoter",

abstract = "We have isolated a 1.6 kb clone from a rat genomic library which contains the bidirectional collagen IV promoter, flanked by exons coding for the α1(IV) and α2(IV) collagen chains. There are at least two transcription start sites within both the α1(IV) and α2(IV)) collagen genes. Rat mesangial cells were transfected with chloramphenicol acetyltransferase (CAT) reporter plasmids containing segments of the promoter and 5' flanking region, in both the α1(IV) and α2(IV) orientations. Our results suggest that transcriptional efficiency of the bidirectional promoter is more efficient in the α2(IV) direction than in the α1(IV) direction.",

keywords = "Basement membrane, Collagen IV, Promoter, Structure",

author = "Grande, {Joseph P.} and Melder, {Deborah C.} and Kluge, {Daniel L.} and Wieben, {Eric D.}",

note = "Funding Information: This work was supported by NIH Grant DK 45280.",

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T1 - Structure of the rat collagen IV promoter

AU - Grande, Joseph P.

AU - Melder, Deborah C.

AU - Kluge, Daniel L.

AU - Wieben, Eric D.

N1 - Funding Information: This work was supported by NIH Grant DK 45280.

PY - 1996/11/11

Y1 - 1996/11/11

N2 - We have isolated a 1.6 kb clone from a rat genomic library which contains the bidirectional collagen IV promoter, flanked by exons coding for the α1(IV) and α2(IV) collagen chains. There are at least two transcription start sites within both the α1(IV) and α2(IV)) collagen genes. Rat mesangial cells were transfected with chloramphenicol acetyltransferase (CAT) reporter plasmids containing segments of the promoter and 5' flanking region, in both the α1(IV) and α2(IV) orientations. Our results suggest that transcriptional efficiency of the bidirectional promoter is more efficient in the α2(IV) direction than in the α1(IV) direction.

AB - We have isolated a 1.6 kb clone from a rat genomic library which contains the bidirectional collagen IV promoter, flanked by exons coding for the α1(IV) and α2(IV) collagen chains. There are at least two transcription start sites within both the α1(IV) and α2(IV)) collagen genes. Rat mesangial cells were transfected with chloramphenicol acetyltransferase (CAT) reporter plasmids containing segments of the promoter and 5' flanking region, in both the α1(IV) and α2(IV) orientations. Our results suggest that transcriptional efficiency of the bidirectional promoter is more efficient in the α2(IV) direction than in the α1(IV) direction.

KW - Basement membrane

KW - Collagen IV

KW - Promoter

KW - Structure

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JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

IS - 1-2

ER -

Structure of the rat collagen IV promoter

Abstract

Keywords

ASJC Scopus subject areas

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