Structure of the gene encoding the human plasma membrane calcium pump isoform 1

H. Hilfiker, M. A. Strehler-Page, T. P. Stauffer, E. Carafoli, E. E. Strehler

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The complete structure of the gene for the human plasma membrane calcium ATPase isoform 1 (hPMCA1) has been elucidated. The protein is encoded by 21 exons present on overlapping clones covering more than 100 kilobases (kb) of DNA. An intron of over 35 kb separates the 5'-untranslated exon 1 from the exon containing the translational start codon. The entire putative promoter and 5'-flanking region is embedded in a CpG island and is characterized by the presence of numerous Sp1 factor-binding sequences and by the absence of a TATA box. In accordance with the ubiquitous tissue distribution of its mRNA these results suggest that the hPMCA1 gene is of the housekeeping type. No alternative splicing comparable to that identified in PMCA2 RNAs at site 'A' and in PMCA3 RNAs close to site 'C' seems to occur in hPMCA1 transcripts; however, a region in intron 6 shows significant resemblance to the site 'A' alternatively spliced exons in PMCA2 and may represent a pseudoexon or a functional exon not yet detected in any PMCA1 mRNA. At six positions, intron interruptions in the hPMCA1 gene correlate with the boundaries of putative transmembrane domains in the protein, whereas most of the remaining intron positions do not show an obvious correlation with the proposed pump domain structure. The limited conservation of intron positions in different P-type pump genes indicates their early separation from a common ancestor.

Original languageEnglish (US)
Pages (from-to)19717-19725
Number of pages9
JournalJournal of Biological Chemistry
Volume268
Issue number26
StatePublished - 1993

Fingerprint

Plasma Membrane Calcium-Transporting ATPases
Gene encoding
Cell membranes
Introns
Exons
Protein Isoforms
Cell Membrane
Pumps
Calcium
Genes
RNA
Messenger RNA
TATA Box
CpG Islands
Initiator Codon
5' Flanking Region
Essential Genes
Alternative Splicing
Tissue Distribution
Conservation

ASJC Scopus subject areas

  • Biochemistry

Cite this

Hilfiker, H., Strehler-Page, M. A., Stauffer, T. P., Carafoli, E., & Strehler, E. E. (1993). Structure of the gene encoding the human plasma membrane calcium pump isoform 1. Journal of Biological Chemistry, 268(26), 19717-19725.

Structure of the gene encoding the human plasma membrane calcium pump isoform 1. / Hilfiker, H.; Strehler-Page, M. A.; Stauffer, T. P.; Carafoli, E.; Strehler, E. E.

In: Journal of Biological Chemistry, Vol. 268, No. 26, 1993, p. 19717-19725.

Research output: Contribution to journalArticle

Hilfiker, H, Strehler-Page, MA, Stauffer, TP, Carafoli, E & Strehler, EE 1993, 'Structure of the gene encoding the human plasma membrane calcium pump isoform 1', Journal of Biological Chemistry, vol. 268, no. 26, pp. 19717-19725.
Hilfiker H, Strehler-Page MA, Stauffer TP, Carafoli E, Strehler EE. Structure of the gene encoding the human plasma membrane calcium pump isoform 1. Journal of Biological Chemistry. 1993;268(26):19717-19725.
Hilfiker, H. ; Strehler-Page, M. A. ; Stauffer, T. P. ; Carafoli, E. ; Strehler, E. E. / Structure of the gene encoding the human plasma membrane calcium pump isoform 1. In: Journal of Biological Chemistry. 1993 ; Vol. 268, No. 26. pp. 19717-19725.
@article{0b968eeb1eeb44a3bc946e7b5a502f6d,
title = "Structure of the gene encoding the human plasma membrane calcium pump isoform 1",
abstract = "The complete structure of the gene for the human plasma membrane calcium ATPase isoform 1 (hPMCA1) has been elucidated. The protein is encoded by 21 exons present on overlapping clones covering more than 100 kilobases (kb) of DNA. An intron of over 35 kb separates the 5'-untranslated exon 1 from the exon containing the translational start codon. The entire putative promoter and 5'-flanking region is embedded in a CpG island and is characterized by the presence of numerous Sp1 factor-binding sequences and by the absence of a TATA box. In accordance with the ubiquitous tissue distribution of its mRNA these results suggest that the hPMCA1 gene is of the housekeeping type. No alternative splicing comparable to that identified in PMCA2 RNAs at site 'A' and in PMCA3 RNAs close to site 'C' seems to occur in hPMCA1 transcripts; however, a region in intron 6 shows significant resemblance to the site 'A' alternatively spliced exons in PMCA2 and may represent a pseudoexon or a functional exon not yet detected in any PMCA1 mRNA. At six positions, intron interruptions in the hPMCA1 gene correlate with the boundaries of putative transmembrane domains in the protein, whereas most of the remaining intron positions do not show an obvious correlation with the proposed pump domain structure. The limited conservation of intron positions in different P-type pump genes indicates their early separation from a common ancestor.",
author = "H. Hilfiker and Strehler-Page, {M. A.} and Stauffer, {T. P.} and E. Carafoli and Strehler, {E. E.}",
year = "1993",
language = "English (US)",
volume = "268",
pages = "19717--19725",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "26",

}

TY - JOUR

T1 - Structure of the gene encoding the human plasma membrane calcium pump isoform 1

AU - Hilfiker, H.

AU - Strehler-Page, M. A.

AU - Stauffer, T. P.

AU - Carafoli, E.

AU - Strehler, E. E.

PY - 1993

Y1 - 1993

N2 - The complete structure of the gene for the human plasma membrane calcium ATPase isoform 1 (hPMCA1) has been elucidated. The protein is encoded by 21 exons present on overlapping clones covering more than 100 kilobases (kb) of DNA. An intron of over 35 kb separates the 5'-untranslated exon 1 from the exon containing the translational start codon. The entire putative promoter and 5'-flanking region is embedded in a CpG island and is characterized by the presence of numerous Sp1 factor-binding sequences and by the absence of a TATA box. In accordance with the ubiquitous tissue distribution of its mRNA these results suggest that the hPMCA1 gene is of the housekeeping type. No alternative splicing comparable to that identified in PMCA2 RNAs at site 'A' and in PMCA3 RNAs close to site 'C' seems to occur in hPMCA1 transcripts; however, a region in intron 6 shows significant resemblance to the site 'A' alternatively spliced exons in PMCA2 and may represent a pseudoexon or a functional exon not yet detected in any PMCA1 mRNA. At six positions, intron interruptions in the hPMCA1 gene correlate with the boundaries of putative transmembrane domains in the protein, whereas most of the remaining intron positions do not show an obvious correlation with the proposed pump domain structure. The limited conservation of intron positions in different P-type pump genes indicates their early separation from a common ancestor.

AB - The complete structure of the gene for the human plasma membrane calcium ATPase isoform 1 (hPMCA1) has been elucidated. The protein is encoded by 21 exons present on overlapping clones covering more than 100 kilobases (kb) of DNA. An intron of over 35 kb separates the 5'-untranslated exon 1 from the exon containing the translational start codon. The entire putative promoter and 5'-flanking region is embedded in a CpG island and is characterized by the presence of numerous Sp1 factor-binding sequences and by the absence of a TATA box. In accordance with the ubiquitous tissue distribution of its mRNA these results suggest that the hPMCA1 gene is of the housekeeping type. No alternative splicing comparable to that identified in PMCA2 RNAs at site 'A' and in PMCA3 RNAs close to site 'C' seems to occur in hPMCA1 transcripts; however, a region in intron 6 shows significant resemblance to the site 'A' alternatively spliced exons in PMCA2 and may represent a pseudoexon or a functional exon not yet detected in any PMCA1 mRNA. At six positions, intron interruptions in the hPMCA1 gene correlate with the boundaries of putative transmembrane domains in the protein, whereas most of the remaining intron positions do not show an obvious correlation with the proposed pump domain structure. The limited conservation of intron positions in different P-type pump genes indicates their early separation from a common ancestor.

UR - http://www.scopus.com/inward/record.url?scp=0027219409&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027219409&partnerID=8YFLogxK

M3 - Article

C2 - 8396145

AN - SCOPUS:0027219409

VL - 268

SP - 19717

EP - 19725

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 26

ER -