TY - JOUR
T1 - Structure and expression of the human polymorphic epithelial mucin gene
T2 - an expressed VNTR unit
AU - Lancaster, Carole A.
AU - Peat, Nigel
AU - Duhig, Trevor
AU - Wilson, David
AU - Taylor-Papadimitriou, Joyce
AU - Gendler, Sandra J.
PY - 1990/12/31
Y1 - 1990/12/31
N2 - The human polymorphic epithelial mucin (PEM) is expressed apically by glandular epithelium and by the carcinomas that develop from these tissues. Previously isolated cDNA clones revealed that the core protein contained a large domain consisting of variable numbers of 60 bp tandem repeats (TR), making it an expressed minisatellite. We now report the full genomic sequence of the PEM gene, including 803 bp of 5′ flanking sequence. The gene is composed of 7 exons and varies in size from ≈4 to ≈7 kb, depending on the number of tandem repeats in exon 2. Expression of PEM was obtained from a genomic clone in an Epstein-Barr virus based vector, after transfection into a human epithelial cell line, indicating the presence of effective regulatory sequences in this clone.
AB - The human polymorphic epithelial mucin (PEM) is expressed apically by glandular epithelium and by the carcinomas that develop from these tissues. Previously isolated cDNA clones revealed that the core protein contained a large domain consisting of variable numbers of 60 bp tandem repeats (TR), making it an expressed minisatellite. We now report the full genomic sequence of the PEM gene, including 803 bp of 5′ flanking sequence. The gene is composed of 7 exons and varies in size from ≈4 to ≈7 kb, depending on the number of tandem repeats in exon 2. Expression of PEM was obtained from a genomic clone in an Epstein-Barr virus based vector, after transfection into a human epithelial cell line, indicating the presence of effective regulatory sequences in this clone.
UR - http://www.scopus.com/inward/record.url?scp=0025551069&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025551069&partnerID=8YFLogxK
U2 - 10.1016/S0006-291X(05)80888-5
DO - 10.1016/S0006-291X(05)80888-5
M3 - Article
C2 - 2268309
AN - SCOPUS:0025551069
SN - 0006-291X
VL - 173
SP - 1019
EP - 1029
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -