Structure and expression of the human polymorphic epithelial mucin gene

an expressed VNTR unit

Carole A. Lancaster, Nigel Peat, Trevor Duhig, David Wilson, Joyce Taylor-Papadimitriou, Sandra J Gendler

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

The human polymorphic epithelial mucin (PEM) is expressed apically by glandular epithelium and by the carcinomas that develop from these tissues. Previously isolated cDNA clones revealed that the core protein contained a large domain consisting of variable numbers of 60 bp tandem repeats (TR), making it an expressed minisatellite. We now report the full genomic sequence of the PEM gene, including 803 bp of 5′ flanking sequence. The gene is composed of 7 exons and varies in size from ≈4 to ≈7 kb, depending on the number of tandem repeats in exon 2. Expression of PEM was obtained from a genomic clone in an Epstein-Barr virus based vector, after transfection into a human epithelial cell line, indicating the presence of effective regulatory sequences in this clone.

Original languageEnglish (US)
Pages (from-to)1019-1029
Number of pages11
JournalBiochemical and Biophysical Research Communications
Volume173
Issue number3
DOIs
StatePublished - Dec 31 1990
Externally publishedYes

Fingerprint

Mucin-1
Tandem Repeat Sequences
Clone Cells
Genes
Exons
Minisatellite Repeats
5' Flanking Region
Human Herpesvirus 4
Viruses
Transfection
Epithelium
Complementary DNA
Epithelial Cells
Tissue
Carcinoma
Cell Line
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Structure and expression of the human polymorphic epithelial mucin gene : an expressed VNTR unit. / Lancaster, Carole A.; Peat, Nigel; Duhig, Trevor; Wilson, David; Taylor-Papadimitriou, Joyce; Gendler, Sandra J.

In: Biochemical and Biophysical Research Communications, Vol. 173, No. 3, 31.12.1990, p. 1019-1029.

Research output: Contribution to journalArticle

Lancaster, Carole A. ; Peat, Nigel ; Duhig, Trevor ; Wilson, David ; Taylor-Papadimitriou, Joyce ; Gendler, Sandra J. / Structure and expression of the human polymorphic epithelial mucin gene : an expressed VNTR unit. In: Biochemical and Biophysical Research Communications. 1990 ; Vol. 173, No. 3. pp. 1019-1029.
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