Structure and chromosome localization of the human eosinophil-derived neurotoxin and eosinophil cationic protein genes: Evidence for intronless coding sequences in the ribonuclease gene superfamily

Kimm J. Hamann, Rosa M. Ten, David A. Loegering, Robert B. Jenkins, Mark T. Heise, Christopher R. Schad, Larry R. Pease, Gerald J. Gleich, Robert L. Barker

Research output: Contribution to journalArticle

93 Scopus citations

Abstract

Human genomic DNAs for the eosinophil granule proteins, eosinophil-derived neurotoxin (EDN) and eosinophil cationic protein (ECP), were isolated from genomic libraries. Alignment of EDN (RNS2) and ECP (RNS3) gene sequences demonstrated remarkable nucleotide similarities in noncoding sequences, introns, and flanking regions, as well as in the previously known coding regions. Detailed examination of the 5′-noncoding regions yielded putative TATA and CAAT boxes, as well as similarities to promoter motifs from unrelated genes. A single intron of 230 bases was found in the 5′ untranslated region and we suggest that a single intron in this region and an intronless coding region are features common to many members of the RNase gene superfamily. The RNS2 and RNS3 genes were localized to the q24-q31 region of human chromosome 14. It is likely that these two genes arose as a consequence of a gene duplication event that took place approximately 25-40 million years ago and that a subset of anthropoid primates possess both of these genes or closely related genes.

Original languageEnglish (US)
Pages (from-to)535-546
Number of pages12
JournalGenomics
Volume7
Issue number4
DOIs
StatePublished - Aug 1990

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'Structure and chromosome localization of the human eosinophil-derived neurotoxin and eosinophil cationic protein genes: Evidence for intronless coding sequences in the ribonuclease gene superfamily'. Together they form a unique fingerprint.

  • Cite this